Literature DB >> 20237646

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.

Ying Huang1, William K Russell, Wei Wan, Pei-Jing Pai, David H Russell, Wenshe Liu.   

Abstract

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

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Year:  2010        PMID： 20237646     DOI： 10.1039/b920120c

Source DB:  PubMed          Journal:  Mol Biosyst        ISSN： 1742-2051


  23 in total

1.  Comparison of strategies for non-perturbing labeling of α-synuclein to study amyloidogenesis.

Authors:  Conor M Haney; Rebecca F Wissner; John B Warner; Yanxin J Wang; John J Ferrie; Dustin J Covell; Richard J Karpowicz; Virginia M-Y Lee; E James Petersson
Journal:  Org Biomol Chem       Date:  2016-02-07       Impact factor: 3.876

Review 2.  Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Authors:  Wei Wan; Jeffery M Tharp; Wenshe R Liu
Journal:  Biochim Biophys Acta       Date:  2014-03-12

3.  A Robust and Quantitative Reporter System To Evaluate Noncanonical Amino Acid Incorporation in Yeast.

Authors:  Jessica T Stieglitz; Haixing P Kehoe; Ming Lei; James A Van Deventer
Journal:  ACS Synth Biol       Date:  2018-09-04       Impact factor: 5.110

Review 4.  At the Interface of Chemical and Biological Synthesis: An Expanded Genetic Code.

Authors:  Han Xiao; Peter G Schultz
Journal:  Cold Spring Harb Perspect Biol       Date:  2016-09-01       Impact factor: 10.005

Review 5.  tRNAPyl: Structure, function, and applications.

Authors:  Jeffery M Tharp; Andreas Ehnbom; Wenshe R Liu
Journal:  RNA Biol       Date:  2017-09-13       Impact factor: 4.652

6.  A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli.

Authors:  Sumana Venkat; Caroline Gregory; Kexin Meng; Qinglei Gan; Chenguang Fan
Journal:  J Vis Exp       Date:  2017-12-09       Impact factor: 1.355

7.  Towards reassigning the rare AGG codon in Escherichia coli.

Authors:  Yu Zeng; Wei Wang; Wenshe R Liu
Journal:  Chembiochem       Date:  2014-07-08       Impact factor: 3.164

Review 8.  Incorporation of Non-Canonical Amino Acids.

Authors:  Lilia Leisle; Francis Valiyaveetil; Ryan A Mehl; Christopher A Ahern
Journal:  Adv Exp Med Biol       Date:  2015       Impact factor: 2.622

9.  Labeling proteins with fluorophore/thioamide Förster resonant energy transfer pairs by combining unnatural amino acid mutagenesis and native chemical ligation.

Authors:  Rebecca F Wissner; Solongo Batjargal; Colin M Fadzen; E James Petersson
Journal:  J Am Chem Soc       Date:  2013-04-17       Impact factor: 15.419

10.  Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion.

Authors:  Ilka U Heinemann; Alexis J Rovner; Hans R Aerni; Svetlana Rogulina; Laura Cheng; William Olds; Jonathan T Fischer; Dieter Söll; Farren J Isaacs; Jesse Rinehart
Journal:  FEBS Lett       Date:  2012-09-13       Impact factor: 4.124
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