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Literature DB >> 20228813

Fluorescence fluctuations of quantum-dot sensors capture intracellular protein interaction dynamics.

Eli Zamir¹, Piet H M Lommerse, Ali Kinkhabwala, Hernán E Grecco, Philippe I H Bastiaens.

Abstract

We extend the in vitro principle of co-immunoprecipitation to quantify dynamic protein interactions in living cells. Using a multiresolution implementation of fluorescence correlation spectroscopy to achieve maximal temporal resolution, we monitored the interactions of endogenous bait proteins, recruited by quantum dots, with fluorescently tagged prey. With this approach, we analyzed the rapid physiological regulation of protein kinase A.

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Year: 2010 PMID： 20228813 DOI： 10.1038/nmeth.1441

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

16 in total

1. Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells.

Authors: Benedict Hebert; Santiago Costantino; Paul W Wiseman
Journal: Biophys J Date: 2005-02-18 Impact factor: 4.033

2. Measuring fast dynamics in solutions and cells with a laser scanning microscope.

Authors: Michelle A Digman; Claire M Brown; Parijat Sengupta; Paul W Wiseman; Alan R Horwitz; Enrico Gratton
Journal: Biophys J Date: 2005-05-20 Impact factor: 4.033

3. Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Authors: Michelle A Digman; Parijat Sengupta; Paul W Wiseman; Claire M Brown; Alan R Horwitz; Enrico Gratton
Journal: Biophys J Date: 2005-03-25 Impact factor: 4.033

Review 4. Reading protein modifications with interaction domains.

Authors: Bruce T Seet; Ivan Dikic; Ming-Ming Zhou; Tony Pawson
Journal: Nat Rev Mol Cell Biol Date: 2006-07 Impact factor: 94.444

5. Delineation of type I protein kinase A-selective signaling events using an RI anchoring disruptor.

Authors: Cathrine Rein Carlson; Birgitte Lygren; Torunn Berge; Naoto Hoshi; Wei Wong; Kjetil Taskén; John D Scott
Journal: J Biol Chem Date: 2006-05-25 Impact factor: 5.157

Review 6. Optical techniques to analyze real-time activation and signaling of G-protein-coupled receptors.

Authors: Martin J Lohse; Viacheslav O Nikolaev; Peter Hein; Carsten Hoffmann; Jean-Pierre Vilardaga; Moritz Bünemann
Journal: Trends Pharmacol Sci Date: 2008-02-11 Impact factor: 14.819

7. Reverse engineering intracellular biochemical networks.

Authors: Eli Zamir; Philippe I H Bastiaens
Journal: Nat Chem Biol Date: 2008-11 Impact factor: 15.040

8. Dissecting cAMP binding domain A in the RIalpha subunit of cAMP-dependent protein kinase. Distinct subsites for recognition of cAMP and the catalytic subunit.

Authors: L J Huang; S S Taylor
Journal: J Biol Chem Date: 1998-10-09 Impact factor: 5.157

9. beta2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics.

Authors: Jonathan D Violin; Lisa M DiPilato; Necmettin Yildirim; Timothy C Elston; Jin Zhang; Robert J Lefkowitz
Journal: J Biol Chem Date: 2007-11-28 Impact factor: 5.157

10. A genetically encoded, fluorescent indicator for cyclic AMP in living cells.

Authors: M Zaccolo; F De Giorgi; C Y Cho; L Feng; T Knapp; P A Negulescu; S S Taylor; R Y Tsien; T Pozzan
Journal: Nat Cell Biol Date: 2000-01 Impact factor: 28.824

5 in total

Review 1. Quantum dots in diagnostics and detection: principles and paradigms.

Authors: T R Pisanic; Y Zhang; T H Wang
Journal: Analyst Date: 2014-04-28 Impact factor: 4.616

2. Probing cellular protein complexes using single-molecule pull-down.

Authors: Ankur Jain; Ruijie Liu; Biswarathan Ramani; Edwin Arauz; Yuji Ishitsuka; Kaushik Ragunathan; Jeehae Park; Jie Chen; Yang K Xiang; Taekjip Ha
Journal: Nature Date: 2011-05-26 Impact factor: 49.962

3. Heterodimerization of β2 adrenergic receptor and somatostatin receptor 5: Implications in modulation of signaling pathway.

Authors: Rishi K Somvanshi; Nicole Chaudhari; Xiaofan Qiu; Ujendra Kumar
Journal: J Mol Signal Date: 2011-08-12

4. Co-imaging extrinsic, intrinsic and effector caspase activity by fluorescence anisotropy microscopy.

Authors: Agustin A Corbat; Klaus C Schuermann; Piotr Liguzinski; Yvonne Radon; Philippe I H Bastiaens; Peter J Verveer; Hernán E Grecco
Journal: Redox Biol Date: 2018-08-09 Impact factor: 11.799

5. Reconceptualizing Fluorescence Correlation Spectroscopy for Monitoring and Analyzing Periodically Passing Objects.

Authors: Eli Zamir; Christoph Frey; Marian Weiss; Silvia Antona; Johannes P Frohnmayer; Jan-Willi Janiesch; Ilia Platzman; Joachim P Spatz
Journal: Anal Chem Date: 2017-10-20 Impact factor: 6.986

5 in total