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Literature DB >> 15792971

Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Michelle A Digman¹, Parijat Sengupta, Paul W Wiseman, Claire M Brown, Alan R Horwitz, Enrico Gratton.

Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

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Year: 2005 PMID： 15792971 PMCID： PMC1305524 DOI： 10.1529/biophysj.105.061788

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

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