| Literature DB >> 10620803 |
M Zaccolo1, F De Giorgi, C Y Cho, L Feng, T Knapp, P A Negulescu, S S Taylor, R Y Tsien, T Pozzan.
Abstract
Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells. By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of cAMP. This new methodology opens the way to the elucidation of the biochemistry of cAMP in vivo.Entities:
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Year: 2000 PMID： 10620803 DOI： 10.1038/71345
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824