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Literature DB >> 2016088

Chromosomal in situ suppression hybridization after Giemsa banding.

M Klever¹, C Grond-Ginsbach, H Scherthan, T M Schroeder-Kurth.

Abstract

We report the successive application of classical Giemsa banding and chromosomal in situ suppression hybridization in clinical cytogenetics. The use of both techniques within one protocol requires an additional fixation of the chromosome preparations and an improved suppression of the labelled repetitive sequences. The combination of these two cytological techniques allows the high resolution mapping of translocated Y-chromosomal sequences in the chromosome set of an XX-male.

Mesh：

Year: 1991 PMID： 2016088 DOI： 10.1007/bf00194638

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

8 in total

1. Interspecies relationship of a repetitive chromosome-specific DNA.

Authors: H Scherthan; S Lutz; R Metzdorf; N Blin
Journal: Hereditas Date: 1989 Impact factor: 3.271

2. Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.

Authors: A Jauch; C Daumer; P Lichter; J Murken; T Schroeder-Kurth; T Cremer
Journal: Hum Genet Date: 1990-07 Impact factor: 4.132

3. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.

Authors: P Lichter; T Cremer; J Borden; L Manuelidis; D C Ward
Journal: Hum Genet Date: 1988-11 Impact factor: 4.132

4. Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.

Authors: T Cremer; P Lichter; J Borden; D C Ward; L Manuelidis
Journal: Hum Genet Date: 1988-11 Impact factor: 4.132

5. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors: D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal: Proc Natl Acad Sci U S A Date: 1988-12 Impact factor: 11.205

6. Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization.

Authors: P M Nederlof; S van der Flier; A K Raap; H J Tanke; M van der Ploeg; F Kornips; J P Geraedts
Journal: Cancer Genet Cytogenet Date: 1989-10-01

Review 7. Counterstain-enhanced chromosome banding.

Authors: D Schweizer
Journal: Hum Genet Date: 1981 Impact factor: 4.132

8. Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.

Authors: J A Garson; J A van den Berghe; J T Kemshead
Journal: Nucleic Acids Res Date: 1987-06-25 Impact factor: 16.971

8 in total

17 in total

1. Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.

Authors: K W Dobie; M Lee; J A Fantes; E Graham; A J Clark; A Springbett; R Lathe; M McClenaghan
Journal: Proc Natl Acad Sci U S A Date: 1996-06-25 Impact factor: 11.205

2. Molecular cytogenetic analysis of XX males using Y-specific DNA sequences, including SRY.

Authors: B Van der Auwera; N Van Roy; A De Paepe; J R Hawkins; I Liebaers; S Castedo; J Dumon; F Speleman
Journal: Hum Genet Date: 1992-04 Impact factor: 4.132

3. Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction.

Authors: I Dunham; C Lengauer; T Cremer; T Featherstone
Journal: Hum Genet Date: 1992-02 Impact factor: 4.132

4. Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries.

Authors: J Wienberg; R Stanyon; A Jauch; T Cremer
Journal: Chromosoma Date: 1992-03 Impact factor: 4.316

Chromosomal in situ suppression hybridization after Giemsa banding.

1. Interspecies relationship of a repetitive chromosome-specific DNA.

2. Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics.

3. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries.

4. Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.

5. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

6. Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization.

Review 7. Counterstain-enhanced chromosome banding.

8. Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.

1. Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.

2. Molecular cytogenetic analysis of XX males using Y-specific DNA sequences, including SRY.

3. Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction.

4. Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries.

5. Sequential G-banding FISH on human sperm chromosomes.

6. A complex chromosomal rearrangement detected prenatally and studied by fluorescence in situ hybridization.

7. Modes of DAPI banding and simultaneous in situ hybridization.

8. Maternal cell contamination in amniotic fluid samples as a consequence of the sampling technique.

9. Reconstruction of genomic rearrangements in great apes and gibbons by chromosome painting.

10. HeLa D98/aH-2 studied by chromosome painting and conventional cytogenetical techniques.