Literature DB >> 9421262

Sequential G-banding FISH on human sperm chromosomes.

P Colls1, C Templado, O Martínez-Pasarell, F Darroudi, A T Natarajan.   

Abstract

A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single- and double-colour chromosome painting was performed using DNA libraries specific for chromosomes X, Y and 21 on either unstained or G-banded preparations. The hybridization efficiency was very high (98%). The sequential staining technique is very useful for analyses of structural (stable) and numerical chromosome aberrations in human sperm and thus can increase the efficiency of the human sperm-hamster oocytes fusion system to assess the risk to human germ cells as a result of endogenous and exogenous factors.

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Year:  1997        PMID： 9421262     DOI： 10.1023/a:1018464929628

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   5.239


  22 in total

1.  Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by in situ hybridization using chromosome-specific DNA libraries.

Authors:  A T Natarajan; R C Vyas; F Darroudi; S Vermeulen
Journal:  Int J Radiat Biol       Date:  1992-02       Impact factor: 2.694

2.  Improved interpretation of complex chromosomal rearrangements by combined GTG banding and in situ suppression hybridization using chromosome-specific libraries and cosmid probes.

Authors:  V T Smit; J W Wessels; P Mollevanger; J G Dauwerse; M van Vliet; G C Beverstock; M H Breuning; P Devilee; A K Raap; C J Cornelisse
Journal:  Genes Chromosomes Cancer       Date:  1991-07       Impact factor: 5.006

3.  Identification of chromosomal structural alterations in human ovarian carcinoma cells using combined GTG-banding and repetitive fluorescence in situ hybridization (FISH).

Authors:  J Xu; N Wang
Journal:  Cancer Genet Cytogenet       Date:  1994-05

4.  Method for sequential staining of GTL-banded metaphases with fluorescent-labeled chromosome-specific paint probes.

Authors:  S M Jalal; M E Law; E R Christensen; J L Spurbeck; G W Dewald
Journal:  Am J Med Genet       Date:  1993-04-01

5.  A detailed method for obtaining preparations of human sperm chromosomes.

Authors:  R H Martin
Journal:  Cytogenet Cell Genet       Date:  1983

6.  Prerequisites for successful human sperm cryobanking: sperm quality and prefreezing holding time.

Authors:  H Yavetz; L Yogev; Z Homonnai; G Paz
Journal:  Fertil Steril       Date:  1991-04       Impact factor: 7.329

7.  Direct chromosomal analysis of human spermatozoa: preliminary results from 18 normal men.

Authors:  R H Martin; C C Lin; W Balkan; K Burns
Journal:  Am J Hum Genet       Date:  1982-05       Impact factor: 11.025

8.  Chromosomal damage in sperm of patients surviving Hodgkin's disease following MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone) therapy with and without radiotherapy.

Authors:  B F Brandriff; M L Meistrich; L A Gordon; A V Carrano; J C Liang
Journal:  Hum Genet       Date:  1994-03       Impact factor: 4.132

9.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

Authors:  D Pinkel; T Straume; J W Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

10.  An increased frequency of human sperm chromosomal abnormalities after radiotherapy.

Authors:  R H Martin; K Hildebrand; J Yamamoto; A Rademaker; M Barnes; G Douglas; K Arthur; T Ringrose; I S Brown
Journal:  Mutat Res       Date:  1986-07       Impact factor: 2.433
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  2 in total

1.  Genetic analysis of meiotic recombination in humans by use of sperm typing: reduced recombination within a heterozygous paracentric inversion of chromosome 9q32-q34.3.

Authors:  G M Brown; M Leversha; M Hulten; M A Ferguson-Smith; N A Affara; R A Furlong
Journal:  Am J Hum Genet       Date:  1998-06       Impact factor: 11.025

2.  Fluorescent in situ hybridization of human sperm: diagnostics, indications, and therapeutic implications.

Authors:  Ranjith Ramasamy; Stefan Besada; Dolores J Lamb
Journal:  Fertil Steril       Date:  2014-10-22       Impact factor: 7.329
  2 in total

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