| Literature DB >> 20079733 |
Qinghua Feng1, Joshua E Stern, Stephen E Hawes, Hiep Lu, Mingjun Jiang, Nancy B Kiviat.
Abstract
Hepatocellular carcinoma (HCC) is known to be associated with both HBV and HCV. While epigenetic changes have been previously reported to be associated with hepatocellular carcinoma (HCC), whether the epigenetic profile of HBC associated HCC differs from that of HCV-associated HCC is unclear. We analyzed DNA methylation of ten genes (APC, CCND2, CDKN2A, GSTP1, HOXA9, RARB, RASSF1, RUNX, SFRP1, and TWIST1) using MethyLight assays on 65 archived liver tissue blocks. Three genes (APC, CCND2, and GSTP1) were frequently methylated in normal liver tissues. Five genes (APC, CDKN2A, HOXA9, RASSF1, and RUNX) were significantly more frequently methylated in malignant liver tissues than normal liver tissues. Among HCC cases, HOXA9, RASSF1 and SFRP1 were methylated more frequently in HBV-positive HCC cases, while CDKN2A were significantly more frequently methylated in HCV-positive HCC cases. Our data support the hypothesis that HCC resulting from different viral etiologies is associated with different epigenetic changes. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20079733 PMCID： PMC2848881 DOI： 10.1016/j.yexmp.2010.01.002
Source DB: PubMed Journal: Exp Mol Pathol ISSN： 0014-4800 Impact factor: 3.362