Literature DB >> 2778063

New fluorescence assay for the quantitation of fungi.

T Coleman1, J V Madassery, G S Kobayashi, M H Nahm, J R Little.   

Abstract

Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.

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Year:  1989        PMID： 2778063      PMCID： PMC267727          DOI： 10.1128/jcm.27.9.2003-2007.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  A comparison of the highly selective fluorescence staining of fungi in tissue sections with Uvitex 2B and Calcofluor White M2R.

Authors:  E D Wachsmuth
Journal:  Histochem J       Date:  1988-04

2.  Encapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans.

Authors:  K J Kwon-Chung; J C Rhodes
Journal:  Infect Immun       Date:  1986-01       Impact factor: 3.441

3.  Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener.

Authors:  H Maeda; N Ishida
Journal:  J Biochem       Date:  1967-08       Impact factor: 3.387

4.  Timing and function of chitin synthesis in yeast.

Authors:  E Cabib; B Bowers
Journal:  J Bacteriol       Date:  1975-12       Impact factor: 3.490

5.  A rapid fluorescent assay to distinguish attached from phagocytized yeast particles.

Authors:  S M Levitz; D J DiBenedetto; R D Diamond
Journal:  J Immunol Methods       Date:  1987-07-16       Impact factor: 2.303

6.  Non-encapsulated variant of Cryptococcus neoformans. II. Surface receptors for cryptococcal polysaccharide and their role in inhibition of phagocytosis by polysaccharide.

Authors:  T R Kozel
Journal:  Infect Immun       Date:  1977-04       Impact factor: 3.441

7.  Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum.

Authors:  K R Klimpel; W E Goldman
Journal:  Infect Immun       Date:  1987-03       Impact factor: 3.441

8.  Cysteine-independent and cysteine-requiring yeast-strains of Histoplasma capsulatum.

Authors:  E S Jacobson; A C Harrell
Journal:  Mycopathologia       Date:  1982-02-19       Impact factor: 2.574

9.  Nonencapsulated Variant of Cryptococcus neoformans I. Virulence Studies and Characterization of Soluble Polysaccharide.

Authors:  T R Kozel; J Cazin
Journal:  Infect Immun       Date:  1971-02       Impact factor: 3.441

10.  Microscopic studies of Candida albicans and Torulopsis glabrata after in vitro treatment with bifonazole. Light and scanning electron microscopy.

Authors:  D Barug; R A Samson; A Kerkenaar
Journal:  Arzneimittelforschung       Date:  1983
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  10 in total

1.  Collaborative comparison of broth macrodilution and microdilution antifungal susceptibility tests.

Authors:  A Espinel-Ingroff; C W Kish; T M Kerkering; R A Fromtling; K Bartizal; J N Galgiani; K Villareal; M A Pfaller; T Gerarden; M G Rinaldi
Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

2.  Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.

Authors:  A Espinel-Ingroff; T M Kerkering
Journal:  J Clin Microbiol       Date:  1991-02       Impact factor: 5.948

3.  Solvatochromic effect in the optical spectra of calcofluor and its relation to fluorescent staining of yeast cell walls.

Authors:  Jaromír Plásek; Barbora Hosková
Journal:  J Fluoresc       Date:  2009-10-31       Impact factor: 2.217

Review 4.  Antifungal susceptibility testing.

Authors:  J H Rex; M A Pfaller; M G Rinaldi; A Polak; J N Galgiani
Journal:  Clin Microbiol Rev       Date:  1993-10       Impact factor: 26.132

Review 5.  Diagnosis of invasive mycoses in severely immunosuppressed patients.

Authors:  R Rüchel
Journal:  Ann Hematol       Date:  1993-07       Impact factor: 3.673

6.  N-acetylglucosamine affects Cryptococcus neoformans cell-wall composition and melanin architecture.

Authors:  Emma Camacho; Christine Chrissian; Radames J B Cordero; Livia Liporagi-Lopes; Ruth E Stark; Arturo Casadevall
Journal:  Microbiology       Date:  2017-10-18       Impact factor: 2.777

7.  Comparison study of broth macrodilution and microdilution antifungal susceptibility tests.

Authors:  A Espinel-Ingroff; T M Kerkering; P R Goldson; S Shadomy
Journal:  J Clin Microbiol       Date:  1991-06       Impact factor: 5.948

8.  Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.

Authors:  Ricardo Perez-Dulzaides; Emma Camacho; Radames J B Cordero; Arturo Casadevall
Journal:  Microbiology       Date:  2018-06-25       Impact factor: 2.777

9.  Rapid protocol for visualization of rust fungi structures using fluorochrome Uvitex 2B.

Authors:  Sheshanka Dugyala; Pawel Borowicz; Maricelis Acevedo
Journal:  Plant Methods       Date:  2015-12-11       Impact factor: 4.993

10.  Characterization of the antifungal functions of a WGA-Fc (IgG2a) fusion protein binding to cell wall chitin oligomers.

Authors:  Susie Coutinho Liedke; Daniel Zamith Miranda; Kamilla Xavier Gomes; Jorge Luis S Gonçalves; Susana Frases; Joshua D Nosanchuk; Marcio L Rodrigues; Leonardo Nimrichter; José Mauro Peralta; Allan J Guimarães
Journal:  Sci Rep       Date:  2017-09-22       Impact factor: 4.379
  10 in total

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