Literature DB >> 20052599

GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro.

Sarah Costers1, David J Lefebvre, Jan Van Doorsselaere, Merijn Vanhee, Peter L Delputte, Hans J Nauwynck.   

Abstract

Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.

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Year:  2010        PMID： 20052599     DOI： 10.1007/s00705-009-0582-7

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  22 in total

1.  Porcine reproductive and respiratory syndrome virus neutralizing antibodies provide in vivo cross-protection to PRRSV1 and PRRSV2 viral challenge.

Authors:  Sally R Robinson; Michael C Rahe; Diem K Gray; Kyra V Martins; Michael P Murtaugh
Journal:  Virus Res       Date:  2018-02-03       Impact factor: 3.303

2.  A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity.

Authors:  Benjamin R Trible; Luca N Popescu; Nicholas Monday; Jay G Calvert; Raymond R R Rowland
Journal:  J Virol       Date:  2015-04-08       Impact factor: 5.103

3.  Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology.

Authors:  Heng Wang; Rongchang Liu; Weidong Zhang; Lingshuang Sun; Zhangyong Ning; Fangxiao Ji; Jin Cui; Guihong Zhang
Journal:  Virus Genes       Date:  2017-06-08       Impact factor: 2.332

4.  Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies.

Authors:  Jia Su; Lei Zhou; Bicheng He; Xinhui Zhang; Xinna Ge; Jun Han; Xin Guo; Hanchun Yang
Journal:  Virol Sin       Date:  2019-07-25       Impact factor: 4.327

5.  A variable region in GP4 of European-type porcine reproductive and respiratory syndrome virus induces neutralizing antibodies against homologous but not heterologous virus strains.

Authors:  Merijn Vanhee; Sarah Costers; Wander Van Breedam; Marc F Geldhof; Jan Van Doorsselaere; Hans J Nauwynck
Journal:  Viral Immunol       Date:  2010-08       Impact factor: 2.257

6.  Genetic relationships of antibody response, viremia level, and weight gain in pigs experimentally infected with porcine reproductive and respiratory syndrome virus1.

Authors:  Andrew S Hess; Ben R Trible; Melanie K Hess; Raymond R Rowland; Joan K Lunney; Graham S Plastow; Jack C M Dekkers
Journal:  J Anim Sci       Date:  2018-09-07       Impact factor: 3.159

7.  Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs.

Authors:  Chul Han An; Salik Nazki; Sung-Chul Park; Yu Jeong Jeong; Ju Huck Lee; Su-Jin Park; Amina Khatun; Won-Il Kim; Youn-Il Park; Jae Cheol Jeong; Cha Young Kim
Journal:  Planta       Date:  2018-01-08       Impact factor: 4.116

8.  Broadening the heterologous cross-neutralizing antibody inducing ability of porcine reproductive and respiratory syndrome virus by breeding the GP4 or M genes.

Authors:  Lei Zhou; Yan-Yan Ni; Pablo Piñeyro; Caitlin M Cossaboom; Sakthivel Subramaniam; Brenton J Sanford; Barbara A Dryman; Yao-Wei Huang; Xiang-Jin Meng
Journal:  PLoS One       Date:  2013-06-24       Impact factor: 3.240

9.  Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing.

Authors:  Jan Van Doorsselaere; Marc Geldhof; Hans J Nauwynck; Peter L Delputte
Journal:  Virol J       Date:  2011-04-10       Impact factor: 4.099

10.  Isolation of Porcine Reproductive and Respiratory Syndrome Virus GP5-Specific, Neutralizing Monoclonal Antibodies From Hyperimmune Sows.

Authors:  Jordan E Young; Cheryl M T Dvorak; Simon P Graham; Michael P Murtaugh
Journal:  Front Immunol       Date:  2021-02-22       Impact factor: 7.561
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