Literature DB >> 20712485

A variable region in GP4 of European-type porcine reproductive and respiratory syndrome virus induces neutralizing antibodies against homologous but not heterologous virus strains.

Merijn Vanhee1, Sarah Costers, Wander Van Breedam, Marc F Geldhof, Jan Van Doorsselaere, Hans J Nauwynck.   

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) can induce severe reproductive failure in sows, and is involved in the porcine respiratory disease complex. The glycoprotein GP4 of the European prototype PRRSV strain Lelystad virus (LV) contains a linear neutralizing epitope that is located in a highly variable region. The current study aimed to evaluate the antibody response against this and other epitopes on GP4 to infection of pigs with European-type PRRSV. It was shown that three virus strains, differing in the region that corresponds to the neutralizing epitope on GP4 of LV, strongly induce antibodies against this area. Antibodies against the epitopes of the different virus strains were purified from polyclonal swine sera, and used in virus-neutralization tests on primary alveolar macrophages. This revealed that antibodies against the variable region in GP4 of different virus strains are able to neutralize infection with homologous but not heterologous virus strains.

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Year:  2010        PMID： 20712485      PMCID： PMC2928701          DOI： 10.1089/vim.2010.0025

Source DB:  PubMed          Journal:  Viral Immunol        ISSN： 0882-8245            Impact factor:   2.257


  56 in total

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