Literature DB >> 20035878

Graphene oxide: a substrate for optimizing preparations of frozen-hydrated samples.

Radosav S Pantelic1, Jannik C Meyer, Ute Kaiser, Wolfgang Baumeister, Jürgen M Plitzko.   

Abstract

Graphene oxide is a hydrophilic derivative of graphene to which biological macromolecules readily attach, with properties superior to those of amorphous carbon films commonly used in electron microscopy. The single-layered crystalline lattice of carbon is highly electron transparent, and exhibits conductivity higher than amorphous carbon. Hence, graphene oxide is a particularly promising substrate for the examination of biological materials by electron microscopy. In this manuscript we compare graphene oxide films to commonly used amorphous carbon films, describing the use of graphene in optimizing the preparation of unstained, vitrified biological macromolecules. (c) 2009 Elsevier Inc. All rights reserved.

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Year:  2009        PMID: 20035878     DOI: 10.1016/j.jsb.2009.12.020

Source DB:  PubMed          Journal:  J Struct Biol        ISSN: 1047-8477            Impact factor:   2.867


  41 in total

1.  Current outcomes when optimizing 'standard' sample preparation for single-particle cryo-EM.

Authors:  B Carragher; Y Cheng; A Frost; R M Glaeser; G C Lander; E Nogales; H-W Wang
Journal:  J Microsc       Date:  2019-10-07       Impact factor: 1.758

2.  What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models.

Authors:  Michael A Cianfrocco; Elizabeth H Kellogg
Journal:  J Chem Inf Model       Date:  2020-03-09       Impact factor: 4.956

Review 3.  Challenges and opportunities in cryo-EM single-particle analysis.

Authors:  Dmitry Lyumkis
Journal:  J Biol Chem       Date:  2019-02-25       Impact factor: 5.157

Review 4.  Cryo-EM in drug discovery: achievements, limitations and prospects.

Authors:  Jean-Paul Renaud; Ashwin Chari; Claudio Ciferri; Wen-Ti Liu; Hervé-William Rémigy; Holger Stark; Christian Wiesmann
Journal:  Nat Rev Drug Discov       Date:  2018-06-08       Impact factor: 84.694

5.  Hierarchical spidroin micellar nanoparticles as the fundamental precursors of spider silks.

Authors:  Lucas R Parent; David Onofrei; Dian Xu; Dillan Stengel; John D Roehling; J Bennett Addison; Christopher Forman; Samrat A Amin; Brian R Cherry; Jeffery L Yarger; Nathan C Gianneschi; Gregory P Holland
Journal:  Proc Natl Acad Sci U S A       Date:  2018-10-22       Impact factor: 11.205

6.  Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit.

Authors:  Alain Scaiola; Cohue Peña; Melanie Weisser; Daniel Böhringer; Marc Leibundgut; Purnima Klingauf-Nerurkar; Stefan Gerhardy; Vikram Govind Panse; Nenad Ban
Journal:  EMBO J       Date:  2018-02-19       Impact factor: 11.598

7.  High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy.

Authors:  Yimo Han; Xiao Fan; Haozhe Wang; Fang Zhao; Christopher G Tully; Jing Kong; Nan Yao; Nieng Yan
Journal:  Proc Natl Acad Sci U S A       Date:  2019-12-26       Impact factor: 11.205

8.  From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.

Authors:  Christoph A Diebolder; Rebecca S Dillard; Ludovic Renault
Journal:  Methods Mol Biol       Date:  2021

9.  A simple approach to characterizing block copolymer assemblies: graphene oxide supports for high contrast multi-technique imaging.

Authors:  Joseph P Patterson; Ana M Sanchez; Nikos Petzetakis; Thomas P Smart; Thomas H Epps; Ian Portman; Neil R Wilson; Rachel K O'Reilly
Journal:  Soft Matter       Date:  2012-03-28       Impact factor: 3.679

Review 10.  Development and application of STEM for the biological sciences.

Authors:  Alioscka A Sousa; Richard D Leapman
Journal:  Ultramicroscopy       Date:  2012-05-18       Impact factor: 2.689

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