Literature DB >> 1992452

Specific initiation by RNA polymerase I in a whole-cell extract from yeast.

M C Schultz1, S Y Choe, R H Reeder.   

Abstract

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.

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Year:  1991        PMID： 1992452      PMCID： PMC50943          DOI： 10.1073/pnas.88.3.1004

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

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Authors:  B Sollner-Webb; J Tower
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2.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

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Authors:  P Labhart; R H Reeder
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4.  Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.

Authors:  M S Klekamp; P A Weil
Journal:  J Biol Chem       Date:  1982-07-25       Impact factor: 5.157

5.  The primary transcript of the ribosomal repeating unit in yeast.

Authors:  J Klootwijk; P de Jonge; R J Planta
Journal:  Nucleic Acids Res       Date:  1979-01       Impact factor: 16.971

6.  An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.

Authors:  R A Koski; D S Allison; M Worthington; B D Hall
Journal:  Nucleic Acids Res       Date:  1982-12-20       Impact factor: 16.971

7.  The major promoter element of rRNA transcription in yeast lies 2 kb upstream.

Authors:  E A Elion; J R Warner
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

8.  Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.

Authors:  A L Greenleaf; J L Kelly; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

9.  The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.

Authors:  R Klemenz; E P Geiduschek
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971

10.  Transcription of an artificial ribosomal RNA gene in yeast.

Authors:  A E Kempers-Veenstra; H van Heerikhuizen; W Musters; J Klootwijk; R J Planta
Journal:  EMBO J       Date:  1984-06       Impact factor: 11.598
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  46 in total

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Authors:  M C Schultz; D J Hockman; T A Harkness; W I Garinther; B A Altheim
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6.  Faithful in vitro transcription by fission yeast RNA polymerase III reveals unique alpha-amanitin sensitivity.

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Authors:  J Saez-Vasquez; C S Pikaard
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-28       Impact factor: 11.205

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Authors:  Miriam E Bucheli; Stephen Buratowski
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9.  Isolation of STD1, a high-copy-number suppressor of a dominant negative mutation in the yeast TATA-binding protein.

Authors:  R W Ganster; W Shen; M C Schmidt
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10.  Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H.

Authors:  Rosalyn C Doepker; Wei-Li Hsu; Holly A Saffran; James R Smiley
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