Literature DB >> 9256430

Chromatin assembly in a yeast whole-cell extract.

M C Schultz1, D J Hockman, T A Harkness, W I Garinther, B A Altheim.   

Abstract

A simple in vitro system that supports chromatin assembly was developed for Saccharomyces cerevisiae. The assembly reaction is ATP-dependent, uses soluble histones and assembly factors, and generates physiologically spaced nucleosomes. We analyze the pathway of histone recruitment into nucleosomes, using this system in combination with genetic methods for the manipulation of yeast. This analysis supports the model of sequential recruitment of H3/H4 tetramers and H2A/H2B dimers into nucleosomes. Using a similar approach, we show that DNA ligase I can play an important role in template repair during assembly. These studies demonstrate the utility of this system for the combined biochemical and genetic analysis of chromatin assembly in yeast.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9256430      PMCID： PMC23016          DOI： 10.1073/pnas.94.17.9034

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  44 in total

1.  Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity.

Authors:  T Fujii-Nakata; Y Ishimi; A Okuda; A Kikuchi
Journal:  J Biol Chem       Date:  1992-10-15       Impact factor: 5.157

2.  Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.

Authors:  J A Kleinschmidt; E Fortkamp; G Krohne; H Zentgraf; W W Franke
Journal:  J Biol Chem       Date:  1985-01-25       Impact factor: 5.157

3.  Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro.

Authors:  T Nelson; R Wiegand; D Brutlag
Journal:  Biochemistry       Date:  1981-04-28       Impact factor: 3.162

4.  Influence of histone H1 on the in vitro replication of DNA and chromatin.

Authors:  L Halmer; C Gruss
Journal:  Nucleic Acids Res       Date:  1995-03-11       Impact factor: 16.971

5.  Chromatin assembly in Xenopus oocytes: in vitro studies.

Authors:  G C Glikin; I Ruberti; A Worcel
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

6.  Folding of 140-base pair length DNA by a core of arginine-rich histones.

Authors:  M Bina-Stein
Journal:  J Biol Chem       Date:  1978-07-25       Impact factor: 5.157

7.  Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.

Authors:  A Verreault; P D Kaufman; R Kobayashi; B Stillman
Journal:  Cell       Date:  1996-10-04       Impact factor: 41.582

8.  The major cytoplasmic histone acetyltransferase in yeast: links to chromatin replication and histone metabolism.

Authors:  M R Parthun; J Widom; D E Gottschling
Journal:  Cell       Date:  1996-10-04       Impact factor: 41.582

9.  Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.

Authors:  R A Laskey; A D Mills; N R Morris
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

10.  Macromolecule synthesis in temperature-sensitive mutants of yeast.

Authors:  L H Hartwell
Journal:  J Bacteriol       Date:  1967-05       Impact factor: 3.490
View more
  24 in total

1.  Rapamycin induces the G0 program of transcriptional repression in yeast by interfering with the TOR signaling pathway.

Authors:  D Zaragoza; A Ghavidel; J Heitman; M C Schultz
Journal:  Mol Cell Biol       Date:  1998-08       Impact factor: 4.272

2.  In vitro assembly of physiological cohesin/DNA complexes.

Authors:  Itay Onn; Douglas Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-13       Impact factor: 11.205

3.  Herpes simplex virus virion host shutoff protein requires a mammalian factor for efficient in vitro endoribonuclease activity.

Authors:  P Lu; F E Jones; H A Saffran; J R Smiley
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

4.  Measuring kinetochore-microtubule interaction in vitro.

Authors:  Jonathan W Driver; Andrew F Powers; Krishna K Sarangapani; Sue Biggins; Charles L Asbury
Journal:  Methods Enzymol       Date:  2014       Impact factor: 1.600

5.  Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates.

Authors:  M E Gelbart; T Rechsteiner; T J Richmond; T Tsukiyama
Journal:  Mol Cell Biol       Date:  2001-03       Impact factor: 4.272

6.  A pipeline for determining protein-protein interactions and proximities in the cellular milieu.

Authors:  Roman I Subbotin; Brian T Chait
Journal:  Mol Cell Proteomics       Date:  2014-08-29       Impact factor: 5.911

7.  Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae.

Authors:  Robert J Scott; C Patrick Lusk; David J Dilworth; John D Aitchison; Richard W Wozniak
Journal:  Mol Biol Cell       Date:  2005-07-06       Impact factor: 4.138

8.  Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.

Authors:  John LaCava; Kelly R Molloy; Martin S Taylor; Michal Domanski; Brian T Chait; Michael P Rout
Journal:  Biotechniques       Date:  2015-03-01       Impact factor: 1.993

9.  Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae.

Authors:  T Tsukiyama; J Palmer; C C Landel; J Shiloach; C Wu
Journal:  Genes Dev       Date:  1999-03-15       Impact factor: 11.361

10.  Herpes simplex virus virion host shutoff protein is stimulated by translation initiation factors eIF4B and eIF4H.

Authors:  Rosalyn C Doepker; Wei-Li Hsu; Holly A Saffran; James R Smiley
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.