| Literature DB >> 19855395 |
Karina B Falbo1, Constance Alabert, Yuki Katou, Su Wu, Junhong Han, Tammy Wehr, Jing Xiao, Xiangwei He, Zhiguo Zhang, Yang Shi, Katsu Shirahige, Philippe Pasero, Xuetong Shen.
Abstract
ATP-dependent chromatin remodeling complexes have been shown to participate in DNA replication in addition to transcription and DNA repair. However, the mechanisms of their involvement in DNA replication remain unclear. Here, we reveal a specific function of the yeast INO80 chromatin remodeling complex in the DNA damage tolerance pathways. Whereas INO80 is necessary for the resumption of replication at forks stalled by methyl methane sulfonate (MMS), it is not required for replication fork collapse after treatment with hydroxyurea (HU). Mechanistically, INO80 regulates DNA damage tolerance during replication through modulation of PCNA (proliferating cell nuclear antigen) ubiquitination and Rad51-mediated processing of recombination intermediates at impeded replication forks. Our findings establish a mechanistic link between INO80 and DNA damage tolerance pathways, indicating that chromatin remodeling is important for accurate DNA replication.Entities:
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Year: 2009 PMID： 19855395 PMCID： PMC2974178 DOI： 10.1038/nsmb.1686
Source DB: PubMed Journal: Nat Struct Mol Biol ISSN： 1545-9985 Impact factor: 15.369