Literature DB >> 19605580

Prevalence and management of invalid GeneXpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study.

Susan E Sefers1, Anna K Raymer, Jessica T Kilby, David H Persing, Yi-Wei Tang.   

Abstract

A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA results were reported for 43 (8.2%) specimens and correlated with increased visibility of red blood cells (P < 0.0001) but not with CSF xanthochromia and clotting. Invalid GXEA result rates were markedly diminished by 82.1% and 96.0% and test sensitivities were minimally decreased by 1.7% and 3.6% when these specimens were tested at a 1:5 dilution and after a freeze-thaw cycle, respectively.

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Year:  2009        PMID： 19605580      PMCID： PMC2738114          DOI： 10.1128/JCM.01238-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

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Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.

Authors:  B Böddinghaus; T A Wichelhaus; V Brade; T Bittner
Journal:  J Clin Microbiol       Date:  2001-10       Impact factor: 5.948

3.  Correlation of cerebrospinal fluid (CSF) cell counts and elevated CSF protein levels with enterovirus reverse transcription-PCR results in pediatric and adult patients.

Authors:  William S Mulford; Richard S Buller; Max Q Arens; Gregory A Storch
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

4.  Multicenter beta trial of the GeneXpert enterovirus assay.

Authors:  Christine B Kost; Beverly Rogers; M Steven Oberste; Christine Robinson; Brenda L Eaves; Kristi Leos; Susan Danielson; Malini Satya; Fred Weir; Frederick S Nolte
Journal:  J Clin Microbiol       Date:  2007-01-24       Impact factor: 5.948

5.  GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels.

Authors:  Katja Seme; Tina Mocilnik; Kristina Fujs Komlos; Ana Doplihar; David H Persing; Mario Poljak
Journal:  J Clin Microbiol       Date:  2008-02-06       Impact factor: 5.948

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Journal:  J Infect Dis       Date:  1990-04       Impact factor: 5.226

7.  Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.

Authors:  A Akane; K Matsubara; H Nakamura; S Takahashi; K Kimura
Journal:  J Forensic Sci       Date:  1994-03       Impact factor: 1.832

8.  Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma.

Authors:  R W Shafer; D J Levee; M A Winters; K L Richmond; D Huang; T C Merigan
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

9.  Enteroviral meningitis in infancy: potential role for polymerase chain reaction in patient management.

Authors:  Y Schlesinger; M H Sawyer; G A Storch
Journal:  Pediatrics       Date:  1994-08       Impact factor: 7.124

10.  Inhibition of human immunodeficiency virus-1 reverse transcriptase by heme and synthetic heme analogs.

Authors:  R Staudinger; N G Abraham; R D Levere; A Kappas
Journal:  Proc Assoc Am Physicians       Date:  1996-01
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  5 in total

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Journal:  Diagn Microbiol Infect Dis       Date:  2017-10-26       Impact factor: 2.803

Review 2.  Molecular methods and platforms for infectious diseases testing a review of FDA-approved and cleared assays.

Authors:  Rajyasree Emmadi; Jerry B Boonyaratanakornkit; Rangaraj Selvarangan; Venkatakrishna Shyamala; Barbara L Zimmer; Laurina Williams; Bonita Bryant; Ted Schutzbank; Michele M Schoonmaker; Jean A Amos Wilson; Leslie Hall; Preeti Pancholi; Kathryn Bernard
Journal:  J Mol Diagn       Date:  2011-08-25       Impact factor: 5.568

3.  Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis.

Authors:  Frederick S Nolte; Beverly B Rogers; Yi-Wei Tang; M Steven Oberste; Christine C Robinson; K Sue Kehl; Kenneth A Rand; Harley A Rotbart; Jose R Romero; Ann-Christine Nyquist; David H Persing
Journal:  J Clin Microbiol       Date:  2010-12-15       Impact factor: 5.948

4.  Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis.

Authors:  Min Lin; Yun-Ran Li; Qi-Wen Lan; Li-Jun Long; Jia-Qi Liu; Ying-Wen Chen; Xun-Jie Cao; Ge-Yuan Wu; Ya-Ping Li; Xu-Guang Guo
Journal:  Ann Clin Microbiol Antimicrob       Date:  2022-06-09       Impact factor: 6.781

Review 5.  Laboratory diagnosis of viral infection.

Authors:  David R Peaper; Marie Louise Landry
Journal:  Handb Clin Neurol       Date:  2014
  5 in total

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