Literature DB >> 11574609

Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.

B Böddinghaus1, T A Wichelhaus, V Brade, T Bittner.   

Abstract

The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.

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Year:  2001        PMID： 11574609      PMCID： PMC88425          DOI： 10.1128/JCM.39.10.3750-3752.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Purification and characterization of PCR-inhibitory components in blood cells.

Authors:  W A Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat.

Authors:  W Abu Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

Review 3.  Standardization and quality control of PCR analyses.

Authors:  H J Burkardt
Journal:  Clin Chem Lab Med       Date:  2000-02       Impact factor: 3.694

4.  Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.

Authors:  U Reischl; N Lehn; H Wolf; L Naumann
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

5.  Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.

Authors:  W A Al-Soud; L J Jönsson; P Râdström
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

6.  Rapid quantification and differentiation of human polyomavirus DNA in undiluted urine from patients after bone marrow transplantation.

Authors:  S S Biel; T K Held; O Landt; M Niedrig; H R Gelderblom; W Siegert; A Nitsche
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

7.  Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid.

Authors:  D Jonas; A Rosenbaum; S Weyrich; S Bhakdi
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

8.  Diagnosis of tuberculosis by Amplicor Mycobacterium tuberculosis test: a multicenter study.

Authors:  E Carpentier; B Drouillard; M Dailloux; D Moinard; E Vallee; B Dutilh; J Maugein; E Bergogne-Berezin; B Carbonnelle
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

9.  Detection of bacterial DNA in cerebrospinal fluid by an assay for simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and streptococci using a seminested PCR strategy.

Authors:  P Rådström; A Bäckman; N Qian; P Kragsbjerg; C Påhlson; P Olcén
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

10.  Screening of respiratory tract specimens for the presence of Mycobacterium tuberculosis by using the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  T Bodmer; A Gurtner; K Schopfer; L Matter
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948
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  21 in total

Review 1.  Molecular diagnostics in tuberculosis.

Authors:  V C C Cheng; W W Yew; K Y Yuen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-11       Impact factor: 3.267

2.  Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.

Authors:  Soumitesh Chakravorty; Jaya Sivaswami Tyagi
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

3.  Prevalence and management of invalid GeneXpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study.

Authors:  Susan E Sefers; Anna K Raymer; Jessica T Kilby; David H Persing; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2009-07-15       Impact factor: 5.948

4.  Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.

Authors:  Sylvie Armand; Pascale Vanhuls; Guy Delcroix; René Courcol; Nadine Lemaître
Journal:  J Clin Microbiol       Date:  2011-03-16       Impact factor: 5.948

5.  Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens.

Authors:  Claudio Piersimoni; Claudio Scarparo; Paola Piccoli; Alessandra Rigon; Giuliana Ruggiero; Domenico Nista; Stefano Bornigia
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

6.  Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.

Authors:  Nancimae Miller; Tim Cleary; Günter Kraus; Andrea K Young; Gina Spruill; H James Hnatyszyn
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

7.  Rapid detection of Mycobacterium tuberculosis in clinical samples by multiplex polymerase chain reaction (mPCR).

Authors:  Thean-Hock Tang; Siti Aminah Ahmed; Mustaffa Musa; Zainul Fadziruddin Zainuddin
Journal:  World J Microbiol Biotechnol       Date:  2013-06-27       Impact factor: 3.312

8.  A sample extraction method for faster, more sensitive PCR-based detection of pathogens in blood culture.

Authors:  John F Regan; Manohar R Furtado; Maxim G Brevnov; Jeanne A Jordan
Journal:  J Mol Diagn       Date:  2012-01-11       Impact factor: 5.568

Review 9.  Nucleic acid testing for tuberculosis at the point-of-care in high-burden countries.

Authors:  Angelika Niemz; David S Boyle
Journal:  Expert Rev Mol Diagn       Date:  2012-09       Impact factor: 5.225

10.  Early diagnosis of extrapulmonary tuberculosis by a new procedure combining broth culture and PCR.

Authors:  Latifa Noussair; Frédéric Bert; Véronique Leflon-Guibout; Nadine Gayet; Marie-Hélène Nicolas-Chanoine
Journal:  J Clin Microbiol       Date:  2009-03-25       Impact factor: 5.948
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