Literature DB >> 18256224

GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels.

Katja Seme1, Tina Mocilnik, Kristina Fujs Komlos, Ana Doplihar, David H Persing, Mario Poljak.   

Abstract

A prospective unblinded comparative evaluation of three assays for the detection of enteroviral RNA performed on 83 positive and 79 negative cerebrospinal fluid samples showed initial and resolved sensitivities of 90.4% and 98.8%, respectively, for the Cepheid GeneXpert enterovirus assay; 94.0% and 97.6%, respectively, for the Argene enterovirus consensus kit; and 100% and 100%, respectively, for an in-house real-time PCR. The initial and resolved specificities were 100% for all three assays.

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Year:  2008        PMID： 18256224      PMCID： PMC2292960          DOI： 10.1128/JCM.01694-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay.

Authors:  W A Verstrepen; S Kuhn; M M Kockx; M E Van De Vyvere; A H Mertens
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Rapid diagnosis of enterovirus infections by real-time PCR on the LightCycler using the TaqMan format.

Authors:  Thomas Watkins-Riedel; Markus Woegerbauer; David Hollemann; Peter Hufnagl
Journal:  Diagn Microbiol Infect Dis       Date:  2002-02       Impact factor: 2.803

3.  Development, technical performance, and clinical evaluation of a NucliSens basic kit application for detection of enterovirus RNA in cerebrospinal fluid.

Authors:  Christine C Ginocchio; Frank Zhang; Amisha Malhotra; Ryhana Manji; Peter Sillekens; Helma Foolen; Marlieke Overdyk; Margot Peeters
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

4.  Real-time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens.

Authors:  Marie L Landry; Robin Garner; David Ferguson
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

5.  A one-step RT-PCR assay using an enzyme-linked detection system for the diagnosis of enterovirus meningitis.

Authors:  K A Stellrecht; I Harding; F M Hussain; N G Mishrik; R T Czap; M L Lepow; R A Venezia
Journal:  J Clin Virol       Date:  2000-09-01       Impact factor: 3.168

6.  Case studies in cost effectiveness of molecular diagnostics for infectious diseases: pulmonary tuberculosis, enteroviral meningitis, and BK virus nephropathy.

Authors:  Frederick S Nolte
Journal:  Clin Infect Dis       Date:  2006-10-31       Impact factor: 9.079

7.  Routine cerebrospinal fluid enterovirus polymerase chain reaction testing reduces hospitalization and antibiotic use for infants 90 days of age or younger.

Authors:  Rebecca L King; Scott A Lorch; Daniela M Cohen; Richard L Hodinka; Keri A Cohn; Samir S Shah
Journal:  Pediatrics       Date:  2007-09       Impact factor: 7.124

8.  Outbreak of neurologic enterovirus type 71 disease: a diagnostic challenge.

Authors:  Carlos M Pérez-Vélez; Marsha S Anderson; Christine C Robinson; Elizabeth J McFarland; W Allan Nix; Mark A Pallansch; M Steven Oberste; Mary P Glodé
Journal:  Clin Infect Dis       Date:  2007-09-13       Impact factor: 9.079

9.  Impact of rapid polymerase chain reaction results on management of pediatric patients with enteroviral meningitis.

Authors:  Christine C Robinson; Mary Willis; Ashley Meagher; Karen E Gieseker; Harley Rotbart; Mary P Glodé
Journal:  Pediatr Infect Dis J       Date:  2002-04       Impact factor: 2.129

10.  Evaluation of a rapid real-time RT-PCR assay for detection of enterovirus RNA in cerebrospinal fluid specimens.

Authors:  W A Verstrepen; P Bruynseels; A H Mertens
Journal:  J Clin Virol       Date:  2002-07       Impact factor: 3.168
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  8 in total

1.  Prevalence and management of invalid GeneXpert enterovirus results obtained with cerebrospinal fluid samples: a 2-year study.

Authors:  Susan E Sefers; Anna K Raymer; Jessica T Kilby; David H Persing; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2009-07-15       Impact factor: 5.948

2.  Comparison of the Cepheid GeneXpert CT/NG assay to the Hologic Aptima Combo2 assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in self-collected rectal swabs.

Authors:  Laura Dize; Barbara Silver; Charlotte Gaydos
Journal:  Diagn Microbiol Infect Dis       Date:  2017-10-26       Impact factor: 2.803

3.  Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis.

Authors:  Frederick S Nolte; Beverly B Rogers; Yi-Wei Tang; M Steven Oberste; Christine C Robinson; K Sue Kehl; Kenneth A Rand; Harley A Rotbart; Jose R Romero; Ann-Christine Nyquist; David H Persing
Journal:  J Clin Microbiol       Date:  2010-12-15       Impact factor: 5.948

4.  Evaluation of GeneXpert EV assay for the rapid diagnosis of enteroviral meningitis: a systematic review and meta-analysis.

Authors:  Min Lin; Yun-Ran Li; Qi-Wen Lan; Li-Jun Long; Jia-Qi Liu; Ying-Wen Chen; Xun-Jie Cao; Ge-Yuan Wu; Ya-Ping Li; Xu-Guang Guo
Journal:  Ann Clin Microbiol Antimicrob       Date:  2022-06-09       Impact factor: 6.781

Review 5.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

6.  Comparison of the genexpert enterovirus assay (GXEA) with real-time one step RT-PCR for the detection of enteroviral RNA in the cerebrospinal fluid of patients with meningitis.

Authors:  JiYoung Hong; Ahyoun Kim; Seoyeon Hwang; Doo-Sung Cheon; Jong-Hyen Kim; June-Woo Lee; Jae-Hak Park; Byunghak Kang
Journal:  Virol J       Date:  2015-02-13       Impact factor: 4.099

Review 7.  Recent advances in diagnostic microbiology.

Authors:  Lulette Tricia C Bravo; Gary W Procop
Journal:  Semin Hematol       Date:  2009-07       Impact factor: 3.851

Review 8.  Point-of-care testing (POCT): Current techniques and future perspectives.

Authors:  Peter B Luppa; Carolin Müller; Alice Schlichtiger; Harald Schlebusch
Journal:  Trends Analyt Chem       Date:  2011-03-21       Impact factor: 12.296
  8 in total

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