Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood.

Literature DB >> 19535529

Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood.

Wendy L J Hansen¹, Cathrien A Bruggeman, Petra F G Wolffs.

Abstract

Two novel preanalysis sample treatment tools were evaluated in combination with four DNA extraction kits for the selective isolation of bacterial DNA from whole blood. The combination of performing a preanalysis sample treatment and using a larger sample volume increased the detection limit to 50 CFU per ml.

Mesh：

Substances：

Year: 2009 PMID： 19535529 PMCID： PMC2725680 DOI： 10.1128/JCM.00821-09

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. Purification and characterization of PCR-inhibitory components in blood cells.

Authors: W A Al-Soud; P Rådström
Journal: J Clin Microbiol Date: 2001-02 Impact factor: 5.948

2. Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR.

Authors: Kaisu Rantakokko-Jalava; Jari Jalava
Journal: J Clin Microbiol Date: 2002-11 Impact factor: 5.948

3. Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Authors: Franziska Zucol; Roland A Ammann; Christoph Berger; Christoph Aebi; Martin Altwegg; Felix K Niggli; David Nadal
Journal: J Clin Microbiol Date: 2006-08 Impact factor: 5.948

Review 4. Advances in nucleic acid-based diagnostics of bacterial infections.

Authors: Kim B Barken; Janus A J Haagensen; Tim Tolker-Nielsen
Journal: Clin Chim Acta Date: 2007-07-13 Impact factor: 3.786

5. Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.

Authors: P Morata; M I Queipo-Ortuño; J de Dios Colmenero
Journal: J Clin Microbiol Date: 1998-09 Impact factor: 5.948

Review 6. Inhibition and facilitation of nucleic acid amplification.

Authors: I G Wilson
Journal: Appl Environ Microbiol Date: 1997-10 Impact factor: 4.792

7. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein.

Authors: C A Kreader
Journal: Appl Environ Microbiol Date: 1996-03 Impact factor: 4.792

8. The population structure of Staphylococcus aureus among general practice patients from The Netherlands.

Authors: G A Donker; R H Deurenberg; C Driessen; S Sebastian; S Nys; E E Stobberingh
Journal: Clin Microbiol Infect Date: 2009-01-28 Impact factor: 8.067

9. Detection and differentiation of in vitro-spiked bacteria by real-time PCR and melting-curve analysis.

Authors: S Klaschik; L E Lehmann; A Raadts; M Book; J Gebel; A Hoeft; F Stuber
Journal: J Clin Microbiol Date: 2004-02 Impact factor: 5.948

10. Selective isolation of bacterial DNA from human clinical specimens.

Authors: Hans-Peter Horz; Sebastian Scheer; Frank Huenger; Morgana E Vianna; Georg Conrads
Journal: J Microbiol Methods Date: 2007-11-28 Impact factor: 2.363

20 in total

1. Improved sensitivity for molecular detection of bacterial and Candida infections in blood.

Authors: Andrea Bacconi; Gregory S Richmond; Michelle A Baroldi; Thomas G Laffler; Lawrence B Blyn; Heather E Carolan; Mark R Frinder; Donna M Toleno; David Metzgar; Jose R Gutierrez; Christian Massire; Megan Rounds; Natalie J Kennel; Richard E Rothman; Stephen Peterson; Karen C Carroll; Teresa Wakefield; David J Ecker; Rangarajan Sampath
Journal: J Clin Microbiol Date: 2014-06-20 Impact factor: 5.948

2. Application of immobilized synthetic anti-lipopolysaccharide peptides for the isolation and detection of bacteria.

Authors: N Sandetskaya; B Engelmann; K Brandenburg; D Kuhlmeier
Journal: Eur J Clin Microbiol Infect Dis Date: 2015-05-19 Impact factor: 3.267

3. Evaluation of MolYsis™ Complete5 DNA extraction method for detecting Staphylococcus aureus DNA from whole blood in a sepsis model using PCR/pyrosequencing.

Authors: Chase D McCann; Jeanne A Jordan
Journal: J Microbiol Methods Date: 2014-02-03 Impact factor: 2.363

Review 4. Developments for improved diagnosis of bacterial bloodstream infections.

Authors: A J M Loonen; P F G Wolffs; C A Bruggeman; A J C van den Brule
Journal: Eur J Clin Microbiol Infect Dis Date: 2014-05-22 Impact factor: 3.267

Review 5. Will polymerase chain reaction (PCR)-based diagnostics improve outcome in septic patients? A clinical view.

Authors: Mathias W Pletz; Nele Wellinghausen; Tobias Welte
Journal: Intensive Care Med Date: 2011-05-15 Impact factor: 17.440

6. Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method.

Authors: M S R Fachmann; C Löfström; J Hoorfar; F Hansen; J Christensen; S Mansdal; M H Josefsen
Journal: Appl Environ Microbiol Date: 2017-02-15 Impact factor: 4.792

7. Molecular probes for diagnosis of clinically relevant bacterial infections in blood cultures.

Authors: Wendy L J Hansen; Judith Beuving; Cathrien A Bruggeman; Petra F G Wolffs
Journal: J Clin Microbiol Date: 2010-10-20 Impact factor: 5.948

8. Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing.

Authors: Matthew Thoendel; Patricio R Jeraldo; Kerryl E Greenwood-Quaintance; Janet Z Yao; Nicholas Chia; Arlen D Hanssen; Matthew P Abdel; Robin Patel
Journal: J Microbiol Methods Date: 2016-05-26 Impact factor: 2.363

9. A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection.

Authors: Peng Zuo; XiuJun Li; Delfina C Dominguez; Bang-Ce Ye
Journal: Lab Chip Date: 2013-10-07 Impact factor: 6.799

10. Improved detection of bacterial central nervous system infections by use of a broad-range PCR assay.

Authors: T Meyer; G Franke; S K A Polywka; M Lütgehetmann; J Gbadamosi; T Magnus; M Aepfelbacher
Journal: J Clin Microbiol Date: 2014-03-12 Impact factor: 5.948