Literature DB >> 18053601

Selective isolation of bacterial DNA from human clinical specimens.

Hans-Peter Horz1, Sebastian Scheer, Frank Huenger, Morgana E Vianna, Georg Conrads.   

Abstract

We evaluated two DNA preparation strategies (MolYsis, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.

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Year:  2007        PMID： 18053601     DOI： 10.1016/j.mimet.2007.10.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  28 in total

1.  Dual priming oligonucleotides for broad-range amplification of the bacterial 16S rRNA gene directly from human clinical specimens.

Authors:  Øyvind Kommedal; Keith Simmon; Dilek Karaca; Nina Langeland; Harald G Wiker
Journal:  J Clin Microbiol       Date:  2012-01-25       Impact factor: 5.948

2.  Evaluation of new preanalysis sample treatment tools and DNA isolation protocols to improve bacterial pathogen detection in whole blood.

Authors:  Wendy L J Hansen; Cathrien A Bruggeman; Petra F G Wolffs
Journal:  J Clin Microbiol       Date:  2009-06-17       Impact factor: 5.948

3.  First experience of a multicenter external quality assessment of molecular 16S rRNA gene detection in bone and joint infections.

Authors:  Chloé Plouzeau; Pascale Bémer; Anne Sophie Valentin; Geneviève Héry-Arnaud; Didier Tandé; Anne Jolivet-Gougeon; Pascal Vincent; Marie Kempf; Carole Lemarié; Jérôme Guinard; Laurent Bret; Anne Sophie Cognée; Sophie Gibaud; Christophe Burucoa; Stéphane Corvec
Journal:  J Clin Microbiol       Date:  2014-11-19       Impact factor: 5.948

4.  Evaluation of MolYsis™ Complete5 DNA extraction method for detecting Staphylococcus aureus DNA from whole blood in a sepsis model using PCR/pyrosequencing.

Authors:  Chase D McCann; Jeanne A Jordan
Journal:  J Microbiol Methods       Date:  2014-02-03       Impact factor: 2.363

Review 5.  Developments for improved diagnosis of bacterial bloodstream infections.

Authors:  A J M Loonen; P F G Wolffs; C A Bruggeman; A J C van den Brule
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-05-22       Impact factor: 3.267

6.  Detection of Salmonella enterica in Meat in Less than 5 Hours by a Low-Cost and Noncomplex Sample Preparation Method.

Authors:  M S R Fachmann; C Löfström; J Hoorfar; F Hansen; J Christensen; S Mansdal; M H Josefsen
Journal:  Appl Environ Microbiol       Date:  2017-02-15       Impact factor: 4.792

7.  Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing.

Authors:  Matthew Thoendel; Patricio R Jeraldo; Kerryl E Greenwood-Quaintance; Janet Z Yao; Nicholas Chia; Arlen D Hanssen; Matthew P Abdel; Robin Patel
Journal:  J Microbiol Methods       Date:  2016-05-26       Impact factor: 2.363

8.  A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi.

Authors:  Liqing Zhou; Andrew J Pollard
Journal:  Ann Clin Microbiol Antimicrob       Date:  2010-04-19       Impact factor: 3.944

9.  Rapid detection of Staphylococcus aureus bacteremia and methicillin resistance by real-time PCR in whole blood samples.

Authors:  N Wellinghausen; D Siegel; S Gebert; J Winter
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2009-03-10       Impact factor: 3.267

10.  Resolvin E1 Reverses Experimental Periodontitis and Dysbiosis.

Authors:  Chun-Teh Lee; Ricardo Teles; Alpdogan Kantarci; Tsute Chen; Jon McCafferty; Jacqueline R Starr; Luciana Carla Neves Brito; Bruce J Paster; Thomas E Van Dyke
Journal:  J Immunol       Date:  2016-08-19       Impact factor: 5.422
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