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Literature DB >> 19470490

Structure of toroidal DNA collapsed inside the phage capsid.

Amélie Leforestier¹, Françoise Livolant.

Abstract

The structure of DNA toroids made of individual DNA molecules of various lengths (3,000 to 55,000 bp) was studied, by using partially filled bacteriophage capsids in conjunction with cryoelectron microscopy. The tetravalent cation spermine was diffused through the capsid to condense the DNA under conditions that were chosen to produce a hexagonal packing. Our results demonstrate that the frustration arising between chirality and hexagonal packing leads to the formation of twist walls; the correlation between helices combined with their strong curvature impose variations of the DNA helical pitch.

Entities: Chemical Species

Mesh：

Substances：
DNA, Viral

Year: 2009 PMID： 19470490 PMCID： PMC2695091 DOI： 10.1073/pnas.0901240106

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

29 in total

Structure of toroidal DNA collapsed inside the phage capsid.

1. EMAN: semiautomated software for high-resolution single-particle reconstructions.

2. Cryoelectron microscopy of lambda phage DNA condensates in vitreous ice: the fine structure of DNA toroids.

3. Controlling the size of nanoscale toroidal DNA condensates with static curvature and ionic strength.

4. Osmotic pressure inhibition of DNA ejection from phage.

5. Defects in chiral columnar phases: Tilt-grain boundaries and iterated moiré maps.

Review 6. DNA condensation by multivalent cations.

7. Crystal structure of the nucleosome core particle at 2.8 A resolution.

8. DNA condensation with polyamines. II. Electron microscopic studies.

9. Cation-induced toroidal condensation of DNA studies with Co3+(NH3)6.

10. Polymer- and salt-induced toroids of hexagonal DNA.

1. Modulating DNA configuration by interfacial traction: an elastic rod model to characterize DNA folding and unfolding.

2. Ion-dependent dynamics of DNA ejections for bacteriophage lambda.

3. Role of DNA-DNA interactions on the structure and thermodynamics of bacteriophages Lambda and P4.

4. Modeling Viral Capsid Assembly.

5. Polymorphism of DNA conformation inside the bacteriophage capsid.

6. Knotting of linear DNA in nano-slits and nano-channels: a numerical study.

7. Topological friction strongly affects viral DNA ejection.

8. Crystalline polymorphism induced by charge regulation in ionic membranes.

9. Local structure of DNA toroids reveals curvature-dependent intermolecular forces.

10. DNA-DNA interactions in bacteriophage capsids are responsible for the observed DNA knotting.