Literature DB >> 19342785

Improvement of crystal quality by surface mutations of beta-lactamase Toho-1.

Tatsuro Shimamura1, Yasushi Nitanai, Takuro Uchiyama, Hiroshi Matsuzawa.   

Abstract

The beta-lactamase Toho-1 exhibits a strong tendency to form merohedrally twinned crystals. Here, the crystal quality of Toho-1 was improved by using surface modification to remove a sulfate ion involved in crystal packing. The surface-modified Toho-1 variant (R274N/R276N) was crystallized under similar conditions to those used for wild-type Toho-1. R274N/R276N did not form merohedrally twinned crystals. The crystals diffracted to a significantly higher resolution (approximately 0.97 A) than the wild-type crystals (1.65 A); they belonged to the same space group and had almost identical unit-cell parameters to those of wild-type Toho-1.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19342785      PMCID： PMC2664765          DOI： 10.1107/S1744309109008240

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  21 in total

1.  Crystal engineering: a case study using the 24 kDa fragment of the DNA gyrase B subunit from Escherichia coli.

Authors:  A D'Arcy; M Stihle; D Kostrewa; G Dale
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-09

2.  An unexpected outcome of surface engineering an integral membrane protein: improved crystallization of cytochrome ba(3) from Thermus thermophilus.

Authors:  Bin Liu; V Mitch Luna; Ying Chen; C David Stout; James A Fee
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-11-21

3.  Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.

Authors:  Lukasz Goldschmidt; David R Cooper; Zygmunt S Derewenda; David Eisenberg
Journal:  Protein Sci       Date:  2007-08       Impact factor: 6.725

4.  Surface-entropy reduction approaches to manipulate crystal forms of beta-ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae.

Authors:  Gopalakrishnan Parthasarathy; Richard Cummings; Joseph W Becker; Stephen M Soisson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2008-01-16

5.  Systematic study on crystal-contact engineering of diphthine synthase: influence of mutations at crystal-packing regions on X-ray diffraction quality.

Authors:  Hisashi Mizutani; K Saraboji; S M Malathy Sony; M N Ponnuswamy; T Kumarevel; B S Krishna Swamy; D K Simanshu; M R N Murthy; Naoki Kunishima
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2008-09-19

6.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

7.  The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.

Authors:  Agnieszka Mateja; Yancho Devedjiev; Daniel Krowarsch; Kenton Longenecker; Zbigniew Dauter; Jacek Otlewski; Zygmunt S Derewenda
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-11-23

8.  Acyl-intermediate structures of the extended-spectrum class A beta-lactamase, Toho-1, in complex with cefotaxime, cephalothin, and benzylpenicillin.

Authors:  Tatsuro Shimamura; Akiko Ibuka; Shinya Fushinobu; Takayoshi Wakagi; Masaji Ishiguro; Yoshikazu Ishii; Hiroshi Matsuzawa
Journal:  J Biol Chem       Date:  2002-09-08       Impact factor: 5.157

9.  Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8 A resolution.

Authors:  A Ibuka; A Taguchi; M Ishiguro; S Fushinobu; Y Ishii; S Kamitori; K Okuyama; K Yamaguchi; M Konno; H Matsuzawa
Journal:  J Mol Biol       Date:  1999-02-05       Impact factor: 5.469

10.  Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli.

Authors:  Y Ishii; A Ohno; H Taguchi; S Imajo; M Ishiguro; H Matsuzawa
Journal:  Antimicrob Agents Chemother       Date:  1995-10       Impact factor: 5.191
View more
  6 in total

1.  Neutron and X-ray crystal structures of a perdeuterated enzyme inhibitor complex reveal the catalytic proton network of the Toho-1 β-lactamase for the acylation reaction.

Authors:  Stephen J Tomanicek; Robert F Standaert; Kevin L Weiss; Andreas Ostermann; Tobias E Schrader; Joseph D Ng; Leighton Coates
Journal:  J Biol Chem       Date:  2012-12-18       Impact factor: 5.157

2.  Probing the role of the conserved residue Glu166 in a class A β-lactamase using neutron and X-ray protein crystallography.

Authors:  Patricia S Langan; Brendan Sullivan; Kevin L Weiss; Leighton Coates
Journal:  Acta Crystallogr D Struct Biol       Date:  2020-01-24       Impact factor: 7.652

3.  Toho-1 β-lactamase: backbone chemical shift assignments and changes in dynamics upon binding with avibactam.

Authors:  Varun V Sakhrani; Rittik K Ghosh; Eduardo Hilario; Kevin L Weiss; Leighton Coates; Leonard J Mueller
Journal:  J Biomol NMR       Date:  2021-07-04       Impact factor: 2.582

4.  Active-Site Protonation States in an Acyl-Enzyme Intermediate of a Class A β-Lactamase with a Monobactam Substrate.

Authors:  Venu Gopal Vandavasi; Patricia S Langan; Kevin L Weiss; Jerry M Parks; Jonathan B Cooper; Stephan L Ginell; Leighton Coates
Journal:  Antimicrob Agents Chemother       Date:  2016-12-27       Impact factor: 5.191

5.  The structure of Toho1 β-lactamase in complex with penicillin reveals the role of Tyr105 in substrate recognition.

Authors:  Patricia S Langan; Venu Gopal Vandavasi; Kevin L Weiss; Jonathan B Cooper; Stephan L Ginell; Leighton Coates
Journal:  FEBS Open Bio       Date:  2016-11-07       Impact factor: 2.693

6.  Protein-peptide complex crystallization: a case study on the ERK2 mitogen-activated protein kinase.

Authors:  Gergő Gógl; Imre Törő; Attila Reményi
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2013-02-16
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.