Literature DB >> 1934108

Ethanol improves the transformation efficiency of intact yeast cells.

V Lauermann1.   

Abstract

A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1934108     DOI： 10.1007/bf00312757

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  20 in total

1.  Specificity of DNA uptake during whole cell transformation of S. cerevisiae.

Authors:  C V Bruschi; A R Comer; G A Howe
Journal:  Yeast       Date:  1987-06       Impact factor: 3.239

2.  Ethanol and the fluidity of the yeast plasma membrane.

Authors:  R P Jones; P F Greenfield
Journal:  Yeast       Date:  1987-12       Impact factor: 3.239

3.  Construction of high copy yeast vectors using 2-microns circle sequences.

Authors:  J R Broach
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

4.  Construction and use of gene fusions to lacZ (beta-galactosidase) that are expressed in yeast.

Authors:  M Rose; D Botstein
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

5.  A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.

Authors:  C S Hoffman; F Winston
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Factors enhancing genetic transformation of intact yeast cells modify cell wall porosity.

Authors:  B Brzobohatý; L Kovác
Journal:  J Gen Microbiol       Date:  1986-11

7.  High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors:  K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Transformation of yeast by a replicating hybrid plasmid.

Authors:  J D Beggs
Journal:  Nature       Date:  1978-09-14       Impact factor: 49.962

9.  A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.

Authors:  R J Klebe; J V Harriss; Z D Sharp; M G Douglas
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

10.  A colony procedure for transformation of Saccharomyces cerevisiae.

Authors:  D Keszenman-Pereyra; K Hieda
Journal:  Curr Genet       Date:  1988       Impact factor: 3.886
View more
  4 in total

1.  SOM 1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae.

Authors:  K Esser; E Pratje; G Michaelis
Journal:  Mol Gen Genet       Date:  1996-09-25

2.  B-function expression in the flower center underlies the homeotic phenotype of Lacandonia schismatica (Triuridaceae).

Authors:  Elena R Álvarez-Buylla; Barbara A Ambrose; Eduardo Flores-Sandoval; Marie Englund; Adriana Garay-Arroyo; Berenice García-Ponce; Eduardo de la Torre-Bárcena; Silvia Espinosa-Matías; Esteban Martínez; Alma Piñeyro-Nelson; Peter Engström; Elliot M Meyerowitz
Journal:  Plant Cell       Date:  2010-11-30       Impact factor: 11.277

Review 3.  Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism.

Authors:  Shigeyuki Kawai; Wataru Hashimoto; Kousaku Murata
Journal:  Bioeng Bugs       Date:  2010 Nov-Dec

4.  Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure.

Authors:  R Soni; J P Carmichael; J A Murray
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.