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Literature DB >> 3319781

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.

Abstract

A procedure for the rapid isolation of DNA from the yeast Saccharomyces cerevisiae is described. To release plasmid DNA for the transformation of Escherichia coli, cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform. Centrifugation of this mixture separates the DNA from cellular debris. E. coli can be efficiently transformed with plasmid present in the aqueous layer without further purification of the plasmid DNA. This procedure also releases chromosomal DNA. Following two ethanol precipitations, the chromosomal DNA can be digested by restriction endonucleases and analysed by Southern blot analysis.

Entities: Chemical Species

Mesh：

Substances：
DNA, Fungal

Year: 1987 PMID： 3319781 DOI： 10.1016/0378-1119(87)90131-4

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

1037 in total

1. The interaction and colocalization of Sam68 with the splicing-associated factor YT521-B in nuclear dots is regulated by the Src family kinase p59(fyn).

Authors: A M Hartmann; O Nayler; F W Schwaiger; A Obermeier; S Stamm
Journal: Mol Biol Cell Date: 1999-11 Impact factor: 4.138

2. Identification of a rice APETALA3 homologue by yeast two-hybrid screening.

Authors: Y H Moon; J Y Jung; H G Kang; G An
Journal: Plant Mol Biol Date: 1999-05 Impact factor: 4.076

3. The Saccharomyces cerevisiae DNA recombination and repair functions of the RAD52 epistasis group inhibit Ty1 transposition.

Authors: A J Rattray; B K Shafer; D J Garfinkel
Journal: Genetics Date: 2000-02 Impact factor: 4.562

4. Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.

Authors: T R Ward; M L Hoang; R Prusty; C K Lau; R L Keil; W L Fangman; B J Brewer
Journal: Mol Cell Biol Date: 2000-07 Impact factor: 4.272

5. Investigation of the relationship between lysogeny and lysis of Lactococcus lactis in cheese using prophage-targeted PCR.

Authors: D O'Sullivan; R P Ross; G F Fitzgerald; A Coffey
Journal: Appl Environ Microbiol Date: 2000-05 Impact factor: 4.792

10. Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR.

Authors: J Z Chen; J Qiu; B Shen; G P Holmquist
Journal: Nucleic Acids Res Date: 2000-09-15 Impact factor: 16.971

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.

1. The interaction and colocalization of Sam68 with the splicing-associated factor YT521-B in nuclear dots is regulated by the Src family kinase p59(fyn).

2. Identification of a rice APETALA3 homologue by yeast two-hybrid screening.

3. The Saccharomyces cerevisiae DNA recombination and repair functions of the RAD52 epistasis group inhibit Ty1 transposition.

4. Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.

5. Investigation of the relationship between lysogeny and lysis of Lactococcus lactis in cheese using prophage-targeted PCR.

6. Cell cycle-dependent binding of yeast heat shock factor to nucleosomes.

7. Identification of TH1 as an interaction partner of A-Raf kinase.

8. Ty3 integrase is required for initiation of reverse transcription.

9. Terminating eukaryote translation: domain 1 of release factor eRF1 functions in stop codon recognition.

10. Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR.