Literature DB >> 6363214

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.

R J Klebe, J V Harriss, Z D Sharp, M G Douglas.   

Abstract

Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated transformation of E. coli is technically simple and yields transformants with an efficiency of 10(6)-10(7) transformants/microgram DNA. Detailed analysis of the parameters involved in PEG-mediated transformation of E. coli reveals basic differences between the PEG and standard CaCl2 methods for transformation of E. coli. PEG-mediated transformation of yeast is far simpler than existing protoplast methods and is comparable in efficiency. The new methods described here for PEG-mediated genetic transformation may prove to be of general utility in performing genetic transformation in a wide variety of organisms.

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Year:  1983        PMID： 6363214     DOI： 10.1016/0378-1119(83)90238-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  141 in total

1.  Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymes.

Authors:  Stefania Brocca; Francesco Secundo; Mattia Ossola; Lilia Alberghina; Giacomo Carrea; Marina Lotti
Journal:  Protein Sci       Date:  2003-10       Impact factor: 6.725

2.  Global regulation of mitochondrial biogenesis in Saccharomyces cerevisiae: ABF1 and CPF1 play opposite roles in regulating expression of the QCR8 gene, which encodes subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase.

Authors:  J H de Winde; L A Grivell
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272

3.  A mutation in the Zn-finger of the GAL4 homolog LAC9 results in glucose repression of its target genes.

Authors:  P Kuger; A Gödecke; K D Breunig
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

4.  A new member of the adenylate kinase family in yeast: PAK3 is highly homologous to mammalian AK3 and is targeted to mitochondria.

Authors:  R Schricker; V Magdolen; W Bandlow
Journal:  Mol Gen Genet       Date:  1992-06

5.  Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene.

Authors:  K A Jolley; E Rapaport; D W Hough; M J Danson; W G Woods; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

6.  AUG codons in the RNA leader sequences of the yeast PET genes CBS1 and SCO1 have no influence on translation efficiency.

Authors:  G Krummeck; T Gottenöf; G Rödel
Journal:  Curr Genet       Date:  1991-12       Impact factor: 3.886

7.  Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene.

Authors:  A L Atkin; K L Roy; J B Bell
Journal:  Mol Cell Biol       Date:  1990-08       Impact factor: 4.272

8.  The function of the hypusine-containing proteins of yeast and other eukaryotes is well conserved.

Authors:  V Magdolen; H Klier; T Wöhl; F Klink; H Hirt; J Hauber; F Lottspeich
Journal:  Mol Gen Genet       Date:  1994-09-28

9.  Cis and trans-acting regulatory elements required for regulation of the CPS1 gene in Saccharomyces cerevisiae.

Authors:  J Bordallo; P Suárez-Rendueles
Journal:  Mol Gen Genet       Date:  1995-03-10

10.  Studies of the role of the propeptides of the Arabidopsis thaliana 2S albumin.

Authors:  K D'Hondt; J Van Damme; C Van Den Bossche; S Leejeerajumnean; R De Rycke; J Derksen; J Vandekerckhove; E Krebbers
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340
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