Literature DB >> 19205763

Development and evaluation of a loop-mediated isothermal amplification method for the rapid detection of Chlamydophila pneumoniae.

Y Kawai1, N Miyashita, F Kishi, M Tabuchi, K Oda, T Yamaguchi, K Kawasaki, T Yamazaki, K Ouchi.   

Abstract

We developed a loop-mediated isothermal amplification (LAMP) method to detect Chlamydophila pneumoniae infection. This assay exclusively amplified C. pneumoniae sequences and no cross-reactivity was observed for other Chlamydia species. The detection limit for this assay was found to be ten elementary bodies in 25 min, as observed in a real-time turbidimeter and electrophoretic analysis. The specificity of the LAMP reaction was confirmed by restriction endonuclease analysis, as well as direct sequencing of the amplified product. Among nasopharyngeal swab specimens from 120 patients with acute respiratory tract infections and 40 healthy individuals, the LAMP results showed 100% agreement with the results of real-time polymerase chain reaction (PCR) assays.

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Year:  2009        PMID： 19205763     DOI： 10.1007/s10096-009-0710-z

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  16 in total

1.  Loop-mediated isothermal amplification of DNA.

Authors:  T Notomi; H Okayama; H Masubuchi; T Yonekawa; K Watanabe; N Amino; T Hase
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

Review 2.  Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada).

Authors:  S F Dowell; R W Peeling; J Boman; G M Carlone; B S Fields; J Guarner; M R Hammerschlag; L A Jackson; C C Kuo; M Maass; T O Messmer; D F Talkington; M L Tondella; S R Zaki
Journal:  Clin Infect Dis       Date:  2001-07-20       Impact factor: 9.079

3.  Comparison of a new quantitative ompA-based real-Time PCR TaqMan assay for detection of Chlamydia pneumoniae DNA in respiratory specimens with four conventional PCR assays.

Authors:  Petra Apfalter; Wolfgang Barousch; Marion Nehr; Athanasios Makristathis; Birgit Willinger; Manfred Rotter; Alexander M Hirschl
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

4.  Real-time turbidimetry of LAMP reaction for quantifying template DNA.

Authors:  Yasuyoshi Mori; Masataka Kitao; Norihiro Tomita; Tsugunori Notomi
Journal:  J Biochem Biophys Methods       Date:  2004-05-31

5.  Clinical differentiation of atypical pneumonia using Japanese guidelines.

Authors:  Tadashi Ishida; Naoyuki Miyashita; Chikara Nakahama
Journal:  Respirology       Date:  2007-01       Impact factor: 6.424

6.  Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae.

Authors:  Mitsuko Seki; Yoshihisa Yamashita; Hirotaka Torigoe; Hiromasa Tsuda; Setsuko Sato; Masao Maeno
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

7.  Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples.

Authors:  Tomotada Iwamoto; Toshiaki Sonobe; Kozaburo Hayashi
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

Review 8.  Chlamydia pneumoniae (TWAR).

Authors:  C C Kuo; L A Jackson; L A Campbell; J T Grayston
Journal:  Clin Microbiol Rev       Date:  1995-10       Impact factor: 26.132

9.  Is it possible to distinguish between atypical pneumonia and bacterial pneumonia?: evaluation of the guidelines for community-acquired pneumonia in Japan.

Authors:  Naoyuki Miyashita; Hiroshi Fukano; Koichiro Yoshida; Yoshihito Niki; Toshiharu Matsushima
Journal:  Respir Med       Date:  2004-10       Impact factor: 3.415

10.  Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus.

Authors:  Thi Cam Thai Hong; Quynh Le Mai; Duc Vuong Cuong; Manmohan Parida; Harumi Minekawa; Tsugunori Notomi; Futoshi Hasebe; Kouichi Morita
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948
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  2 in total

1.  Molecular Detection of New Delhi Metallo-Beta-Lactamase-1 (NDM-1) Positive Bacteria from Environmental and Drinking Water Samples by Loop Mediated Isothermal Amplification of bla NDM-1.

Authors:  P Rathinasabapathi; Deepak S Hiremath; Rex Arunraj; M Parani
Journal:  Indian J Microbiol       Date:  2015-06-24       Impact factor: 2.461

2.  Development and evaluation of rapid novel isothermal amplification assays for important veterinary pathogens: Chlamydia psittaci and Chlamydia pecorum.

Authors:  Martina Jelocnik; Md Mominul Islam; Danielle Madden; Cheryl Jenkins; James Branley; Scott Carver; Adam Polkinghorne
Journal:  PeerJ       Date:  2017-09-08       Impact factor: 2.984
  2 in total

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