Literature DB >> 19194012

Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.

Jiansong Ju1, Jianxun Qi, Shujing Xu, Kouhei Ohnishi, Michael J Benedik, Yanfen Xue, Yanhe Ma.   

Abstract

Alanine racemase (DadX(OF4)), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P2(1)2(1)2(1), with two protein molecules in the asymmetric unit.

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Year:  2009        PMID： 19194012      PMCID： PMC2635857          DOI： 10.1107/S174430910900013X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

1.  Purification, properties, and partial amino acid sequences of alanine racemase from the muscle of the black tiger prawn Penaeus monodon.

Authors:  Naoko Yoshikawa; Naoshi Dhomae; Koji Takio; Hiroki Abe
Journal:  Comp Biochem Physiol B Biochem Mol Biol       Date:  2002-11       Impact factor: 2.231

Review 2.  Enzymes in the D-alanine branch of bacterial cell wall peptidoglycan assembly.

Authors:  C T Walsh
Journal:  J Biol Chem       Date:  1989-02-15       Impact factor: 5.157

3.  Characterization of endogenous pyridoxal 5'-phosphate-dependent alanine racemase from Bacillus pseudofirmus OF4.

Authors:  Jiansong Ju; Shujing Xu; Jianxin Wen; Gang Li; Kouhei Ohnishi; Yanfen Xue; Yanhe Ma
Journal:  J Biosci Bioeng       Date:  2009-03       Impact factor: 2.894

4.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

5.  Functional characterization of alanine racemase from Schizosaccharomyces pombe: a eucaryotic counterpart to bacterial alanine racemase.

Authors:  T Uo; T Yoshimura; N Tanaka; K Takegawa; N Esaki
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

6.  Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4.

Authors:  H Takami; T A Krulwich
Journal:  Extremophiles       Date:  2000-02       Impact factor: 2.395

7.  Role of tyrosine 265 of alanine racemase from Bacillus stearothermophilus.

Authors:  A Watanabe; Y Kurokawa; T Yoshimura; N Esaki
Journal:  J Biochem       Date:  1999-06       Impact factor: 3.387

8.  A gene encoding alanine racemase is involved in spore germination in Bacillus thuringiensis.

Authors:  Xiaohua Yan; Yuling Gai; Liang Liang; Gang Liu; Huarong Tan
Journal:  Arch Microbiol       Date:  2006-12-13       Impact factor: 2.552

9.  Alanine racemase of alfalfa seedlings (Medicago sativa L.): first evidence for the presence of an amino acid racemase in plants.

Authors:  Kazutoshi Ono; Kazuki Yanagida; Tadao Oikawa; Tadashi Ogawa; Kenji Soda
Journal:  Phytochemistry       Date:  2006-04-17       Impact factor: 4.072

10.  Purification and preliminary crystallization of alanine racemase from Streptococcus pneumoniae.

Authors:  Ulrich Strych; Milya Davlieva; Joseph P Longtin; Eileen L Murphy; Hookang Im; Michael J Benedik; Kurt L Krause
Journal:  BMC Microbiol       Date:  2007-05-17       Impact factor: 3.605
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  1 in total

1.  Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26.

Authors:  Junlin Liu; Lei Feng; Yawei Shi; Wei Feng
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-09-29
  1 in total

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