Literature DB >> 23027757

Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26.

Junlin Liu1, Lei Feng, Yawei Shi, Wei Feng.   

Abstract

A recombinant form of alanine racemase (Alr) from Pseudomonas putida YZ-26 has been crystallized by the sitting-drop vapour diffusion method. X-ray diffraction data were collected to 2.4 Å resolution. The crystals belong to the space group C222(1), with unit-cell parameters a = 118.08, b = 141.86, c = 113.83 Å, and contain an Alr dimer in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.8 Å(3) Da(-1) and approximately 50%, respectively.

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Year:  2012        PMID： 23027757      PMCID： PMC3497987          DOI： 10.1107/S1744309112036974

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  12 in total

1.  Effect of a Y265F mutant on the transamination-based cycloserine inactivation of alanine racemase.

Authors:  Timothy D Fenn; Todd Holyoak; Geoffrey F Stamper; Dagmar Ringe
Journal:  Biochemistry       Date:  2005-04-12       Impact factor: 3.162

2.  Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases.

Authors:  Jiansong Ju; Shujing Xu; Yukio Furukawa; Yong Zhang; Haruo Misono; Tohru Minamino; Keiichi Namba; Baohua Zhao; Kouhei Ohnishi
Journal:  J Biochem       Date:  2010-10-22       Impact factor: 3.387

3.  Alanine racemase from Helicobacter pylori NCTC 11637: purification, characterization and gene cloning.

Authors:  Makoto Saito; Katsushi Nishimura; Yuichiro Hasegawa; Tomomitsu Shinohara; Sadao Wakabayashi; Tatsuo Kurihara; Morio Ishizuka; Yoko Nagata
Journal:  Life Sci       Date:  2006-12-28       Impact factor: 5.037

4.  Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.

Authors:  U Strych; H C Huang; K L Krause; M J Benedik
Journal:  Curr Microbiol       Date:  2000-10       Impact factor: 2.188

5.  Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution.

Authors:  J P Shaw; G A Petsko; D Ringe
Journal:  Biochemistry       Date:  1997-02-11       Impact factor: 3.162

6.  The 1.9 A crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site.

Authors:  Pierre LeMagueres; Hookang Im; Jerry Ebalunode; Ulrich Strych; Michael J Benedik; James M Briggs; Harold Kohn; Kurt L Krause
Journal:  Biochemistry       Date:  2005-02-08       Impact factor: 3.162

7.  Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P).

Authors:  Kinfai Au; Jingshan Ren; Thomas S Walter; Karl Harlos; Joanne E Nettleship; Raymond J Owens; David I Stuart; Robert M Esnouf
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-04-05

8.  Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae. Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine.

Authors:  Masafumi Noda; Yumi Kawahara; Azusa Ichikawa; Yasuyuki Matoba; Hiroaki Matsuo; Dong-Geun Lee; Takanori Kumagai; Masanori Sugiyama
Journal:  J Biol Chem       Date:  2004-08-09       Impact factor: 5.157

9.  Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4.

Authors:  Jiansong Ju; Jianxun Qi; Shujing Xu; Kouhei Ohnishi; Michael J Benedik; Yanfen Xue; Yanhe Ma
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-01-31

10.  Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50).

Authors:  Emma R Scaletti; Sylvia R Luckner; Kurt L Krause
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-12-09
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