PURPOSE: To determine the effects of visible light on development of mouse embryos and the potential of fibroblast cells to overcome deleterious effects of visible light on mouse preimplantation stage embryos. METHODS: Two-cell mouse embryos were randomly allocated to un-exposed group (control) and exposed group receiving 1600 lx visible light for various time lengths. Both exposed and un-exposed embryos were co-cultured with either Mouse Embryonic Fibroblast (MEF) or Human Embryonic Fibroblast (HEF). Developmental rate of embryos at day 3 (morula), 4 (expanded blastocyst) and 5 (hatching or hatched blastocyst) was evaluated. RESULTS: Exposure of embryos to visible light for 30 min decreased developmental rate significantly (P<0.01). Developmental rate of exposed embryos co-cultured with MEF (58%; p<0.05 both at day 4 and 5) and HEF (67%; P<0.01 both at day 4 and 5) was higher than control. CONCLUSIONS: Visible light adversely affects embryo development in a time-dependent manner. Feeder cells may enhance embryo development particularly when suboptimal conditions are involved.
PURPOSE: To determine the effects of visible light on development of mouse embryos and the potential of fibroblast cells to overcome deleterious effects of visible light on mouse preimplantation stage embryos. METHODS: Two-cell mouse embryos were randomly allocated to un-exposed group (control) and exposed group receiving 1600 lx visible light for various time lengths. Both exposed and un-exposed embryos were co-cultured with either Mouse Embryonic Fibroblast (MEF) or Human Embryonic Fibroblast (HEF). Developmental rate of embryos at day 3 (morula), 4 (expanded blastocyst) and 5 (hatching or hatched blastocyst) was evaluated. RESULTS: Exposure of embryos to visible light for 30 min decreased developmental rate significantly (P<0.01). Developmental rate of exposed embryos co-cultured with MEF (58%; p<0.05 both at day 4 and 5) and HEF (67%; P<0.01 both at day 4 and 5) was higher than control. CONCLUSIONS: Visible light adversely affects embryo development in a time-dependent manner. Feeder cells may enhance embryo development particularly when suboptimal conditions are involved.
Authors: S Hatoya; Y Sugiyama; R Torii; V Wijewardana; D Kumagai; K Sugiura; K Kida; N Kawate; H Tamada; T Sawada; T Inaba Journal: Theriogenology Date: 2006-04-18 Impact factor: 2.740
Authors: József Bódis; Krisztina Gödöny; Ákos Várnagy; Kálmán Kovács; Miklós Koppán; Bernadett Nagy; János Erostyák; Róbert Herczeg; Julia Szekeres-Barthó; Attila Gyenesei; Gabor L Kovács Journal: Med Princ Pract Date: 2020-05-29 Impact factor: 1.927