S G d'Estaing1, J Lornage, S Hadj, D Boulieu, B Salle, J F Guérin. 1. Département de Médecine de la Reproduction, Centre hospitalier de l'Université de Biologie de la reproduction, Hôpital Edouard Herriot, Lyon, France. sge@rockefeller.univ-lyon1.fr
Abstract
OBJECTIVE: To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period. DESIGN: Retrospective study. SETTING: Institutional practice assisted reproductive technology center. PATIENT(S): Ninety-nine selected patients undergoing IVF with blastocyst culture. INTERVENTION(S): In vitro fertilization and transfer of at least one expanded blastocyst after culture using either Vero cells or sequential media (S(2) medium). MAIN OUTCOME MEASURE(S): Blastocyst, pregnancy, and implantation rates. RESULT(S): The blastocyst formation rate (41.6% vs. 31.6%) is significantly increased with sequential media compared with the coculture system, but there is no statistically significant difference in the pregnancy and implantation rates (39.5% vs. 38.6% and 23.7% vs. 15.1%, respectively). CONCLUSION(S): Blastocyst culture on sequential media offers a better dynamic of embryo development (22% vs. 13.6% blastocyst at day 5). There was no statistically significant difference between the two groups in the take-home baby rate (18 vs. 12).
OBJECTIVE: To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period. DESIGN: Retrospective study. SETTING: Institutional practice assisted reproductive technology center. PATIENT(S): Ninety-nine selected patients undergoing IVF with blastocyst culture. INTERVENTION(S): In vitro fertilization and transfer of at least one expanded blastocyst after culture using either Vero cells or sequential media (S(2) medium). MAIN OUTCOME MEASURE(S): Blastocyst, pregnancy, and implantation rates. RESULT(S): The blastocyst formation rate (41.6% vs. 31.6%) is significantly increased with sequential media compared with the coculture system, but there is no statistically significant difference in the pregnancy and implantation rates (39.5% vs. 38.6% and 23.7% vs. 15.1%, respectively). CONCLUSION(S): Blastocyst culture on sequential media offers a better dynamic of embryo development (22% vs. 13.6% blastocyst at day 5). There was no statistically significant difference between the two groups in the take-home baby rate (18 vs. 12).