PURPOSE: To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells). METHODS: We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase-polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos. RESULTS: The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage. CONCLUSIONS: KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system.
PURPOSE: To evaluate the effect on the coculture of murine embryos with a humanovarian granulosa tumor derived cell line (KGN cells). METHODS: We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase-polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos. RESULTS: The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage. CONCLUSIONS: KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system.
Authors: A Hanisch; K D Dieterich; K Dietzmann; K Lüdecke; M Buchfelder; R Fahlbusch; H Lehnert Journal: J Clin Endocrinol Metab Date: 2000-11 Impact factor: 5.958
Authors: C Sáez; A C González-Baena; M A Japón; J Giráldez; D I Segura; J M Rodríguez-Vallejo; J González-Esteban; G Miranda; F Torrubia Journal: Prostate Date: 1999-07-01 Impact factor: 4.104
Authors: Y Nishi; T Yanase; Y Mu; K Oba; I Ichino; M Saito; M Nomura; C Mukasa; T Okabe; K Goto; R Takayanagi; Y Kashimura; M Haji; H Nawata Journal: Endocrinology Date: 2001-01 Impact factor: 4.736