Literature DB >> 1909348

Differentiation of Bacillus anthracis from Bacillus cereus by gas chromatographic whole-cell fatty acid analysis.

D Lawrence1, S Heitefuss, H S Seifert.   

Abstract

Three strains of Bacillus anthracis and seven strains of Bacillus cereus were grown on complex medium and on synthetic medium. Gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. Fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. Significant differences between the fatty acid patterns of the two species were found. Odd iso/anteiso fatty acid ratios were about equal in B. anthracis strains, whereas in B. cereus strains the fractions of iso acids were at least twice as high as the fractions of anteiso acids. The method described herein is used in our diagnostic laboratory to help differentiate between these two species.

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Year:  1991        PMID： 1909348      PMCID： PMC270143          DOI： 10.1128/jcm.29.7.1508-1512.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  [Bacterial differential diagnosis of the anthrax by means of a new specific test called the string-of perls-test].

Authors:  J JENSEN; H KLEEMEYER
Journal:  Zentralbl Bakteriol Orig       Date:  1953-09-05

Review 2.  Fatty acids of the genus Bacillus: an example of branched-chain preference.

Authors:  T Kaneda
Journal:  Bacteriol Rev       Date:  1977-06

3.  [A new method to classify pathogene anaerobes, the statistical evaluation of quantitative determined patterns from metabolic fatty acids (author's transl)].

Authors:  H S Seifert; H Böhnel; J Bunge; A Erbrecht; U von Kuenheim; H Weck
Journal:  Zentralbl Bakteriol Orig A       Date:  1979-03

4.  Selective medium for Bacillus anthracis.

Authors:  R F Knisely
Journal:  J Bacteriol       Date:  1966-09       Impact factor: 3.490

5.  Production and purification of anthrax toxin.

Authors:  S H Leppla
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

6.  Identification of Bacillus strains using the API system.

Authors:  N A Logan; R C Berkeley
Journal:  J Gen Microbiol       Date:  1984-07

7.  Factors affecting the relative ratio of fatty acids in Bacillus cereus.

Authors:  T Kaneda
Journal:  Can J Microbiol       Date:  1971-02       Impact factor: 2.419

8.  Differential media for the identification of Bacillus anthracis.

Authors:  R F Knisely
Journal:  J Bacteriol       Date:  1965-12       Impact factor: 3.490

9.  Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species.

Authors:  T Kaneda
Journal:  J Bacteriol       Date:  1967-03       Impact factor: 3.490

10.  Fatty acids in the genus Bacillus. II. Similarity in the fatty acid compositions of Bacillus thuringiensis, Bacillus anthracis, and Bacillus cereus.

Authors:  T Kaneda
Journal:  J Bacteriol       Date:  1968-06       Impact factor: 3.490
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  5 in total

1.  Subtyping of Bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction.

Authors:  G M Matar; T A Slieman; N H Nabbut
Journal:  Eur J Epidemiol       Date:  1996-06       Impact factor: 8.082

2.  Determination of carbohydrate profiles of Bacillus anthracis and Bacillus cereus including identification of O-methyl methylpentoses by using gas chromatography-mass spectrometry.

Authors:  A Fox; G E Black; K Fox; S Rostovtseva
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

3.  Use of fatty acid methyl ester profiles for discrimination of Bacillus cereus T-strain spores grown on different media.

Authors:  Christopher J Ehrhardt; Vivian Chu; TeeCie Brown; Terrie L Simmons; Brandon K Swan; Jason Bannan; James M Robertson
Journal:  Appl Environ Microbiol       Date:  2010-01-22       Impact factor: 4.792

4.  Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains.

Authors:  R Brousseau; A Saint-Onge; G Préfontaine; L Masson; J Cabana
Journal:  Appl Environ Microbiol       Date:  1993-01       Impact factor: 4.792

Review 5.  Bacillus cereus and related species.

Authors:  F A Drobniewski
Journal:  Clin Microbiol Rev       Date:  1993-10       Impact factor: 26.132
  5 in total

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