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Literature DB >> 19053789

High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.

Gopal Iyer¹, Xavier Michalet, Yun-Pei Chang, Fabien F Pinaud, Stephanie E Matyas, Gregory Payne, Shimon Weiss.

Abstract

We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.

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Year: 2008 PMID： 19053789 PMCID： PMC3084663 DOI： 10.1021/nl8032284

Source DB: PubMed Journal: Nano Lett ISSN： 1530-6984 Impact factor: 11.189

16 in total

Review 1. Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.

Authors: X Michalet; T D Lacoste; S Weiss
Journal: Methods Date: 2001-09 Impact factor: 3.608

2. Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and proteasome degradation.

Authors: J Ma; S Lindquist
Journal: Proc Natl Acad Sci U S A Date: 2001-12-11 Impact factor: 11.205

3. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.

Authors: E T Boder; K S Midelfort; K D Wittrup
Journal: Proc Natl Acad Sci U S A Date: 2000-09-26 Impact factor: 11.205

4. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

Authors: Maxime Dahan; Sabine Lévi; Camilla Luccardini; Philippe Rostaing; Béatrice Riveau; Antoine Triller
Journal: Science Date: 2003-10-17 Impact factor: 47.728

Review 5. Site-specific labeling of proteins with small molecules in live cells.

Authors: Irwin Chen; Alice Y Ting
Journal: Curr Opin Biotechnol Date: 2005-02 Impact factor: 9.740

Review 6. Quantum dots for live cells, in vivo imaging, and diagnostics.

Authors: X Michalet; F F Pinaud; L A Bentolila; J M Tsay; S Doose; J J Li; G Sundaresan; A M Wu; S S Gambhir; S Weiss
Journal: Science Date: 2005-01-28 Impact factor: 47.728

Review 7. Advances in single-molecule fluorescence methods for molecular biology.

Authors: Chirlmin Joo; Hamza Balci; Yuji Ishitsuka; Chittanon Buranachai; Taekjip Ha
Journal: Annu Rev Biochem Date: 2008 Impact factor: 23.643

Review 8. The green fluorescent protein.

Authors: R Y Tsien
Journal: Annu Rev Biochem Date: 1998 Impact factor: 23.643

9. Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.

Authors: Michael J Feldhaus; Robert W Siegel; Lee K Opresko; James R Coleman; Jane M Weaver Feldhaus; Yik A Yeung; Jennifer R Cochran; Peter Heinzelman; David Colby; Jeffrey Swers; Christilyn Graff; H Steven Wiley; K Dane Wittrup
Journal: Nat Biotechnol Date: 2003-01-21 Impact factor: 54.908

10. Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides.

Authors: Fabien Pinaud; David King; Hsiao-Ping Moore; Shimon Weiss
Journal: J Am Chem Soc Date: 2004-05-19 Impact factor: 15.419

13 in total

1. Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures.

Authors: Christopher I Richards; Khai Luong; Rahul Srinivasan; Stephen W Turner; Dennis A Dougherty; Jonas Korlach; Henry A Lester
Journal: Nano Lett Date: 2012-06-08 Impact factor: 11.189

Review 2. Probing cellular events, one quantum dot at a time.

Authors: Fabien Pinaud; Samuel Clarke; Assa Sittner; Maxime Dahan
Journal: Nat Methods Date: 2010-03-30 Impact factor: 28.547

3. Compact biocompatible quantum dots via RAFT-mediated synthesis of imidazole-based random copolymer ligand.

Authors: Wenhao Liu; Andrew B Greytak; Jungmin Lee; Cliff R Wong; Jongnam Park; Lisa F Marshall; Wen Jiang; Peter N Curtin; Alice Y Ting; Daniel G Nocera; Dai Fukumura; Rakesh K Jain; Moungi G Bawendi
Journal: J Am Chem Soc Date: 2010-01-20 Impact factor: 15.419

High affinity scFv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells.

Review 1. Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes.

2. Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and proteasome degradation.

3. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.

4. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

Review 5. Site-specific labeling of proteins with small molecules in live cells.

Review 6. Quantum dots for live cells, in vivo imaging, and diagnostics.

Review 7. Advances in single-molecule fluorescence methods for molecular biology.

Review 8. The green fluorescent protein.

9. Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.

10. Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides.

1. Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures.

Review 2. Probing cellular events, one quantum dot at a time.

3. Compact biocompatible quantum dots via RAFT-mediated synthesis of imidazole-based random copolymer ligand.

4. Tracking single proteins in live cells using single-chain antibody fragment-fluorescent quantum dot affinity pair.

Review 5. Quantum dots find their stride in single molecule tracking.

6. Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging.

7. Stable, compact, bright biofunctional quantum dots with improved peptide coating.

8. Single-quantum dot imaging with a photon counting camera.

9. Labeling Cytosolic Targets in Live Cells with Blinking Probes.

10. Tracking Single Molecules in Biomembranes: Is Seeing Always Believing?