| Literature DB >> 19053413 |
Ségolène Laage1, Alessandro Marchetti, Julien Sein, Roberta Pierattelli, Hans Juergen Sass, Stephan Grzesiek, Anne Lesage, Guido Pintacuda, Lyndon Emsley.
Abstract
A magic angle spinning (MAS) NMR technique to transfer polarization from protons to a specific set of the (13)C spins is introduced for the study of biomolecular samples in the solid-state. Ultrafast (>60 kHz) MAS and low irradiation rf fields are used to achieve band-selective Hartmann-Hahn cross-polarization (CP) between the whole proton bath and carbons whose resonances are close to the (13)C-transmitter offset. When compared to conventional, broadband (1)H-(13)C CP, the band-selective experiment can be established without any loss of sensitivity when polarizing the aliphatic signals of a protein sample, and with a significant gain when polarizing carbonyls. This scheme can be used as a building block in 2D (13)C-(13)C homonuclear correlation experiments to obtain a faster and more sensitive characterization of biological solids.Entities:
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Year: 2008 PMID： 19053413 DOI： 10.1021/ja805926d
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419