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Literature DB >> 19028869

Dpb11 activates the Mec1-Ddc2 complex.

Daniel A Mordes¹, Edward A Nam, David Cortez.

Abstract

The Saccharomyces cerevisiae Mec1-Ddc2 checkpoint kinase complex (the ortholog to human ATR-ATRIP) is an essential regulator of genomic integrity. The S. cerevisiae BRCT repeat protein Dpb11 functions in the initiation of both DNA replication and cell cycle checkpoints. Here, we report a genetic and physical interaction between Dpb11 and Mec1-Ddc2. A C-terminal domain of Dpb11 is sufficient to associate with Mec1-Ddc2 and strongly stimulates the kinase activity of Mec1 in a Ddc2-dependent manner. Furthermore, Mec1 phosphorylates Dpb11 and thereby amplifies the stimulating effect of Dpb11 on Mec1-Ddc2 kinase activity. Thus, Dpb11 is a functional ortholog of human TopBP1, and the Mec1/ATR activation mechanism is conserved from yeast to humans.

Entities: Gene Species

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Year: 2008 PMID： 19028869 PMCID： PMC2596233 DOI： 10.1073/pnas.0806621105

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

31 in total

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Authors: John Rouse; Stephen P Jackson
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2. Two checkpoint complexes are independently recruited to sites of DNA damage in vivo.

Authors: J A Melo; J Cohen; D P Toczyski
Journal: Genes Dev Date: 2001-11-01 Impact factor: 11.361

3. A DNA damage-regulated BRCT-containing protein, TopBP1, is required for cell survival.

Authors: Kazuhiko Yamane; Xianglin Wu; Junjie Chen
Journal: Mol Cell Biol Date: 2002-01 Impact factor: 4.272

Review 4. ATR: an essential regulator of genome integrity.

Authors: Karlene A Cimprich; David Cortez
Journal: Nat Rev Mol Cell Biol Date: 2008-07-02 Impact factor: 94.444

5. Pie1, a protein interacting with Mec1, controls cell growth and checkpoint responses in Saccharomyces cerevisiae.

Authors: T Wakayama; T Kondo; S Ando; K Matsumoto; K Sugimoto
Journal: Mol Cell Biol Date: 2001-02 Impact factor: 4.272

6. LCD1: an essential gene involved in checkpoint control and regulation of the MEC1 signalling pathway in Saccharomyces cerevisiae.

Authors: J Rouse; S P Jackson
Journal: EMBO J Date: 2000-11-01 Impact factor: 11.598

7. Recruitment of Mec1 and Ddc1 checkpoint proteins to double-strand breaks through distinct mechanisms.

Authors: T Kondo; T Wakayama; T Naiki; K Matsumoto; K Sugimoto
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8. Substrate specificities and identification of putative substrates of ATM kinase family members.

Authors: S T Kim; D S Lim; C E Canman; M B Kastan
Journal: J Biol Chem Date: 1999-12-31 Impact factor: 5.157

9. Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint.

Authors: Jerzy Majka; Peter M J Burgers
Journal: Proc Natl Acad Sci U S A Date: 2003-02-25 Impact factor: 11.205

10. Genetic and physical interactions between DPB11 and DDC1 in the yeast DNA damage response pathway.

Authors: Hong Wang; Stephen J Elledge
Journal: Genetics Date: 2002-04 Impact factor: 4.562

67 in total

1. The unstructured C-terminal tail of yeast Dpb11 (human TopBP1) protein is dispensable for DNA replication and the S phase checkpoint but required for the G2/M checkpoint.

Authors: Vasundhara M Navadgi-Patil; Sandeep Kumar; Peter M Burgers
Journal: J Biol Chem Date: 2011-09-28 Impact factor: 5.157

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Authors: Lyne Khair; Ya-Ting Chang; Lakxmi Subramanian; Paul Russell; Toru M Nakamura
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3. Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response.

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Review 4. DNA damage sensing by the ATM and ATR kinases.

Authors: Alexandre Maréchal; Lee Zou
Journal: Cold Spring Harb Perspect Biol Date: 2013-09-01 Impact factor: 10.005

5. Coupling of human DNA excision repair and the DNA damage checkpoint in a defined in vitro system.

Authors: Laura A Lindsey-Boltz; Michael G Kemp; Joyce T Reardon; Vanessa DeRocco; Ravi R Iyer; Paul Modrich; Aziz Sancar
Journal: J Biol Chem Date: 2014-01-08 Impact factor: 5.157

6. Analysis of mutations that dissociate G(2) and essential S phase functions of human ataxia telangiectasia-mutated and Rad3-related (ATR) protein kinase.

Authors: Edward A Nam; Runxiang Zhao; David Cortez
Journal: J Biol Chem Date: 2011-09-09 Impact factor: 5.157

7. Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway.

Authors: Yiyong Liu; Yanan Fang; Hongbing Shao; Laura Lindsey-Boltz; Aziz Sancar; Paul Modrich
Journal: J Biol Chem Date: 2009-12-22 Impact factor: 5.157

Review 8. RPA-coated single-stranded DNA as a platform for post-translational modifications in the DNA damage response.

Authors: Alexandre Maréchal; Lee Zou
Journal: Cell Res Date: 2014-11-18 Impact factor: 25.617

9. The unstructured C-terminal tail of the 9-1-1 clamp subunit Ddc1 activates Mec1/ATR via two distinct mechanisms.

Authors: Vasundhara M Navadgi-Patil; Peter M Burgers
Journal: Mol Cell Date: 2009-12-11 Impact factor: 17.970

10. A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase of Saccharomyces cerevisiae.

Authors: Ryan Janke; Kristina Herzberg; Michael Rolfsmeier; Jordan Mar; Vladimir I Bashkirov; Edwin Haghnazari; Greg Cantin; John R Yates; Wolf-Dietrich Heyer
Journal: Nucleic Acids Res Date: 2010-01-08 Impact factor: 16.971