Literature DB >> 18937951

Evaluation of a rapid method for the quantitative analysis of fatty acids in various matrices.

Pedro Araujo1, Thu-Thao Nguyen, Livar Frøyland, Jingdong Wang, Jing X Kang.   

Abstract

A simplified method for quantitative analysis of fatty acids in various matrices by gas chromatography is proposed as an alternative to the conventional method and the variables of the protocol examined to optimize the processing conditions. The modified method involves direct methylation of fatty acids in homogenized samples with boron trihalide (BF(3) or BCl(3) in methanol) followed by extraction with hexane. The addition of hexane to the reaction mixture after the methylation process can enhance the efficiency of fatty acid methylation and is critical for those samples that contain high levels of triglycerides. A mechanism underlying this effect is proposed.

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Year:  2008        PMID： 18937951      PMCID： PMC2593112          DOI： 10.1016/j.chroma.2008.10.006

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  8 in total

1.  A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples.

Authors:  M Sönnichsen; B W Müller
Journal:  Lipids       Date:  1999-12       Impact factor: 1.880

2.  Selection of methylation procedures for quantitation of short-chain glycerol-bound compounds formed during thermoxidation.

Authors:  O Berdeaux; G Marquez-Ruiz; C Dobarganes
Journal:  J Chromatogr A       Date:  1999-11-26       Impact factor: 4.759

3.  Direct methylation from mouse plasma and from liver and brain homogenates.

Authors:  Nobuya Shirai; Hiramitsu Suzuki; Shun Wada
Journal:  Anal Biochem       Date:  2005-08-01       Impact factor: 3.365

4.  Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues.

Authors:  Sonnich Meier; Svein A Mjøs; Horaldur Joensen; Otto Grahl-Nielsen
Journal:  J Chromatogr A       Date:  2005-12-15       Impact factor: 4.759

5.  A simplified and efficient method for the analysis of fatty acid methyl esters suitable for large clinical studies.

Authors:  Athar Masood; Ken D Stark; Norman Salem
Journal:  J Lipid Res       Date:  2005-08-01       Impact factor: 5.922

6.  Comparison of four extraction/methylation analytical methods to measure fatty acid composition by gas chromatography in meat.

Authors:  M Juárez; O Polvillo; M Contò; A Ficco; S Ballico; S Failla
Journal:  J Chromatogr A       Date:  2008-03-07       Impact factor: 4.759

7.  Comparison of rapid methods for analysis of bacterial fatty acids.

Authors:  C W Moss; M A Lambert; W H Merwin
Journal:  Appl Microbiol       Date:  1974-07

8.  A simplified method for analysis of polyunsaturated fatty acids.

Authors:  Jing X Kang; Jingdong Wang
Journal:  BMC Biochem       Date:  2005-03-24       Impact factor: 4.059
  8 in total
  37 in total

1.  Correcting the effects of -20 °C storage and aliquot size on erythrocyte fatty acid content in the Women's Health Initiative.

Authors:  James V Pottala; Mark A Espeland; Jason Polreis; Jennifer Robinson; William S Harris
Journal:  Lipids       Date:  2012-07-11       Impact factor: 1.880

2.  Erythrocyte fatty acid composition of Nepal breast-fed infants.

Authors:  Sigrun Henjum; Øyvind Lie; Manjeswori Ulak; Andrew L Thorne-Lyman; Ram K Chandyo; Prakash S Shrestha; Wafaie W Fawzi; Tor A Strand; Marian Kjellevold
Journal:  Eur J Nutr       Date:  2017-02-25       Impact factor: 5.614

3.  Comprehensive analysis of the major lipid classes in sebum by rapid resolution high-performance liquid chromatography and electrospray mass spectrometry.

Authors:  Emanuela Camera; Matteo Ludovici; Marisa Galante; Jo-Linda Sinagra; Mauro Picardo
Journal:  J Lipid Res       Date:  2010-08-17       Impact factor: 5.922

4.  Fish consumption, sleep, daily functioning, and heart rate variability.

Authors:  Anita L Hansen; Lisbeth Dahl; Gina Olson; David Thornton; Ingvild E Graff; Livar Frøyland; Julian F Thayer; Staale Pallesen
Journal:  J Clin Sleep Med       Date:  2014-05-15       Impact factor: 4.062

5.  Discrimination of n-3 rich oils by gas chromatography.

Authors:  Pedro Araujo; Yingxu Zeng; Zhen-Yu Du; Thu-Thao Nguyen; Livar Frøyland; Bjørn Grung
Journal:  Lipids       Date:  2010-10-21       Impact factor: 1.880

6.  GC-EI-MS analysis of fatty acid composition in brain and serum of twitcher mouse.

Authors:  Assunta Zanfini; Elena Dreassi; Anna Berardi; Paola Piomboni; Elvira Costantino-Ceccarini; Alice Luddi
Journal:  Lipids       Date:  2014-09-11       Impact factor: 1.880

7.  Improved extraction of saturated fatty acids but not omega-3 fatty acids from sheep red blood cells using a one-step extraction procedure.

Authors:  Edward H Clayton; Catherine E Gulliver; John W Piltz; Robert D Taylor; Robert J Blake; Richard G Meyer
Journal:  Lipids       Date:  2012-05-10       Impact factor: 1.880

8.  Plasma fatty acid ratios affect blood gene expression profiles--a cross-sectional study of the Norwegian Women and Cancer Post-Genome Cohort.

Authors:  Karina Standahl Olsen; Christopher Fenton; Livar Frøyland; Marit Waaseth; Ruth H Paulssen; Eiliv Lund
Journal:  PLoS One       Date:  2013-06-25       Impact factor: 3.240

9.  Fatty acid variability in three medicinal herbs of Panax species.

Authors:  Xiao-Jing Zhang; Li-Li Huang; Xiu-Jiang Cai; Peng Li; Yi-Tao Wang; Jian-Bo Wan
Journal:  Chem Cent J       Date:  2013-01-21       Impact factor: 4.215

10.  Survey of n-3 and n-6 polyunsaturated fatty acids in fish and fish products.

Authors:  Claudia Strobel; Gerhard Jahreis; Katrin Kuhnt
Journal:  Lipids Health Dis       Date:  2012-10-30       Impact factor: 3.876
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