Literature DB >> 10652996

A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples.

M Sönnichsen1, B W Müller.   

Abstract

A quantitative method for the one-step esterification and determination of absolute amounts of polyunsaturated fatty acids in biotechnologically produced fungal mycelia is proposed. A system of two internal standards was used to quantify the total fatty acid content of the samples by gas chromatography. The degree of methylation of the analyzed fatty acids was calculated using the internal standards, and subsequently this quotient was used to validate the derivatization and extraction reactions. By utilizing this degree of methylation, the corrected amounts of the various fatty acids in the sample can be calculated.

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Year:  1999        PMID： 10652996     DOI： 10.1007/s11745-999-0488-z

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  6 in total

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5.  Fatty acid production characteristics of fungi with particular emphasis on gamma linolenic acid production.

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6.  Gamma linolenic acid (GLA) content of encapsulated evening primrose oil products.

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Journal:  Lipids       Date:  1992-01       Impact factor: 1.880
  6 in total
  8 in total

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Review 2.  Development in lipid analysis: some new extraction techniques and in situ transesterification.

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  8 in total

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