Literature DB >> 18930011

Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity.

Akinori Matsushika1, Shigeki Sawayama.   

Abstract

We varied the promoter strength of xylose reductase (XR) gene and the copy number of xylulokinase (XK) gene to determine how XR and XK activities affect the xylose-fermenting abilities of recombinant Saccharomyces cerevisiae expressing xylitol dehydrogenase (XDH). The most enhanced ethanol yield and lowered xylitol yield occurred in strain I-PGK/AUR, which has high activity of both XR and XDH and moderate XK activity.

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Year:  2008        PMID： 18930011     DOI： 10.1263/jbb.106.306

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  19 in total

1.  Functional Analysis of Two l-Arabinose Transporters from Filamentous Fungi Reveals Promising Characteristics for Improved Pentose Utilization in Saccharomyces cerevisiae.

Authors:  Jingen Li; Jing Xu; Pengli Cai; Bang Wang; Yanhe Ma; J Philipp Benz; Chaoguang Tian
Journal:  Appl Environ Microbiol       Date:  2015-04-03       Impact factor: 4.792

2.  Enhanced expression of genes involved in initial xylose metabolism and the oxidative pentose phosphate pathway in the improved xylose-utilizing Saccharomyces cerevisiae through evolutionary engineering.

Authors:  Jian Zha; Minghua Shen; Menglong Hu; Hao Song; Yingjin Yuan
Journal:  J Ind Microbiol Biotechnol       Date:  2013-10-11       Impact factor: 3.346

3.  An improved method of xylose utilization by recombinant Saccharomyces cerevisiae.

Authors:  Tien-Yang Ma; Ting-Hsiang Lin; Teng-Chieh Hsu; Chiung-Fang Huang; Gia-Luen Guo; Wen-Song Hwang
Journal:  J Ind Microbiol Biotechnol       Date:  2012-06-28       Impact factor: 3.346

4.  A genetic overhaul of Saccharomyces cerevisiae 424A(LNH-ST) to improve xylose fermentation.

Authors:  Aloke K Bera; Nancy W Y Ho; Aftab Khan; Miroslav Sedlak
Journal:  J Ind Microbiol Biotechnol       Date:  2010-08-17       Impact factor: 3.346

5.  Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization.

Authors:  Stefan Krahulec; Barbara Petschacher; Michael Wallner; Karin Longus; Mario Klimacek; Bernd Nidetzky
Journal:  Microb Cell Fact       Date:  2010-03-10       Impact factor: 5.328

6.  Deletion of FPS1, encoding aquaglyceroporin Fps1p, improves xylose fermentation by engineered Saccharomyces cerevisiae.

Authors:  Na Wei; Haiqing Xu; Soo Rin Kim; Yong-Su Jin
Journal:  Appl Environ Microbiol       Date:  2013-03-08       Impact factor: 4.792

7.  Efficient bioethanol production by a recombinant flocculent Saccharomyces cerevisiae strain with a genome-integrated NADP+-dependent xylitol dehydrogenase gene.

Authors:  Akinori Matsushika; Hiroyuki Inoue; Seiya Watanabe; Tsutomu Kodaki; Keisuke Makino; Shigeki Sawayama
Journal:  Appl Environ Microbiol       Date:  2009-03-27       Impact factor: 4.792

8.  D-Xylulose kinase from Saccharomyces cerevisiae: isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli.

Authors:  Simone L Pival; Ruth Birner-Gruenberger; Corinna Krump; Bernd Nidetzky
Journal:  Protein Expr Purif       Date:  2011-06-02       Impact factor: 1.650

9.  Balance of XYL1 and XYL2 expression in different yeast chassis for improved xylose fermentation.

Authors:  Jian Zha; Meng-Long Hu; Ming-Hua Shen; Bing-Zhi Li; Jing-Yu Wang; Ying-Jin Yuan
Journal:  Front Microbiol       Date:  2012-10-05       Impact factor: 5.640

10.  Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered Saccharomyces cerevisiae.

Authors:  Jian Zha; Bing-Zhi Li; Ming-Hua Shen; Meng-Long Hu; Hao Song; Ying-Jin Yuan
Journal:  PLoS One       Date:  2013-07-02       Impact factor: 3.240
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