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Literature DB >> 1886616

Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17.

C Cunningham¹, C A McPherson, J Martin, W J Harris, H J Flint.

Abstract

A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethyl-cellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

Entities: Chemical Species

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Year: 1991 PMID： 1886616 DOI： 10.1007/bf00282484

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

16 in total

Review 1. Molecular biology of cellulose degradation.

Authors: P Béguin
Journal: Annu Rev Microbiol Date: 1990 Impact factor: 15.500

2. Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda.

Authors: G T Howard; B A White
Journal: Appl Environ Microbiol Date: 1988-07 Impact factor: 4.792

10. Preparation of the cellulase from the cellulolytic anaerobic rumen bacterium Ruminococcus albus and its release from the bacterial cell wall.

Authors: T M Wood; C A Wilson; C S Stewart
Journal: Biochem J Date: 1982-07-01 Impact factor: 3.857

4 in total

1. Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria.

Authors: T R Whitehead
Journal: Curr Microbiol Date: 1993-07 Impact factor: 2.188

2. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

Authors: B Henrissat; A Bairoch
Journal: Biochem J Date: 1993-08-01 Impact factor: 3.857

3. EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.

Authors: M T Rincón; S I McCrae; J Kirby; K P Scott; H J Flint
Journal: Appl Environ Microbiol Date: 2001-10 Impact factor: 4.792

4. Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens.

Authors: J X Zhang; J Martin; H J Flint
Journal: Mol Gen Genet Date: 1994-10-28

4 in total

Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17.

Review 1. Molecular biology of cellulose degradation.

2. Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda.

3. Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.

4. Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity.

5. Expression of two xylanase genes from the rumen cellulolytic bacterium Ruminococcus flavefaciens 17 cloned in pUC13.

6. Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1.

7. The properties of forms of Ruminococcus flavefaciens which differ in their ability to degrade cotton cellulose.

8. Studies on transformation of Escherichia coli with plasmids.

9. Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene.

10. Preparation of the cellulase from the cellulolytic anaerobic rumen bacterium Ruminococcus albus and its release from the bacterial cell wall.

1. Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria.

2. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

3. EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.

4. Identification of non-catalytic conserved regions in xylanases encoded by the xynB and xynD genes of the cellulolytic rumen anaerobe Ruminococcus flavefaciens.