Literature DB >> 2687251

Structure of a Ruminococcus albus endo-1,4-beta-glucanase gene.

K Ohmiya1, T Kajino, A Kato, S Shimizu.   

Abstract

A chromosomal DNA fragment encoding an endo-1,4-beta-glucanase I (Eg I) gene from Ruminococcus albus cloned and expressed in Escherichia coli with pUC18 was fully sequenced by the dideoxy-chain termination method. The sequence contained a consensus promoter sequence and a structural amino acid sequence. The initial 43 amino acids of the protein were deduced to be a signal sequence, since they are missing in the mature protein (Eg I). High homology was found when the amino acid sequence of the Eg I was compared with that of endoglucanase E from Clostridium thermocellum. Codon usage of the gene was not biased. These results suggested that the properties of the Eg I gene from R. albus was specified from the known beta-glucanase genes of the other organisms.

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Year:  1989        PMID: 2687251      PMCID: PMC210575          DOI: 10.1128/jb.171.12.6771-6775.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  28 in total

1.  Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B.

Authors:  O Grépinet; P Béguin
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

2.  Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.

Authors:  F Fukumori; N Sashihara; T Kudo; K Horikoshi
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

3.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

4.  Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.

Authors:  P Béguin; P Cornet; J P Aubert
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

5.  Purification and properties of cellobiosidase from Ruminococcus albus.

Authors:  K Ohmiya; M Shimizu; M Taya; S Shimizu
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

6.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

7.  Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi.

Authors:  W K Wong; B Gerhard; Z M Guo; D G Kilburn; A J Warren; R C Miller
Journal:  Gene       Date:  1986       Impact factor: 3.688

8.  Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.

Authors:  L M Robson; G H Chambliss
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

9.  Evidence for two functional gal promoters in intact Escherichia coli cells.

Authors:  H Aiba; S Adhya; B de Crombrugghe
Journal:  J Biol Chem       Date:  1981-11-25       Impact factor: 5.157

10.  Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp.

Authors:  H Zappe; W A Jones; D T Jones; D R Woods
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

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  12 in total

Review 1.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

2.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

3.  EndB, a multidomain family 44 cellulase from Ruminococcus flavefaciens 17, binds to cellulose via a novel cellulose-binding module and to another R. flavefaciens protein via a dockerin domain.

Authors:  M T Rincón; S I McCrae; J Kirby; K P Scott; H J Flint
Journal:  Appl Environ Microbiol       Date:  2001-10       Impact factor: 4.792

4.  Nucleotide sequence of the Ruminococcus albus SY3 endoglucanase genes celA and celB.

Authors:  D M Poole; G P Hazlewood; J I Laurie; P J Barker; H J Gilbert
Journal:  Mol Gen Genet       Date:  1990-09

5.  Three surface layer homology domains at the N terminus of the Clostridium cellulovorans major cellulosomal subunit EngE.

Authors:  Y Tamaru; R H Doi
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

6.  Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase.

Authors:  L Zhou; G P Xue; C G Orpin; G W Black; H J Gilbert; G P Hazlewood
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

7.  Transcriptional induction and expression of the endoglucanase celA gene from a ruminal Clostridium sp. ("C. longisporum").

Authors:  V Mittendorf; J A Thomson
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

8.  Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.

Authors:  O Matsushita; J B Russell; D B Wilson
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

9.  Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17.

Authors:  C Cunningham; C A McPherson; J Martin; W J Harris; H J Flint
Journal:  Mol Gen Genet       Date:  1991-08

10.  Modification of the properties of a Ruminococcus albus endo-1,4-beta-glucanase by gene truncation.

Authors:  K Ohmiya; H Deguchi; S Shimizu
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

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