Literature DB >> 7763664

Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria.

T R Whitehead1.   

Abstract

The DNA sequence for the xylanase gene from Prevotella (Bacteroides) ruminicola 23 was determined. The xylanase gene encoded for a protein with a molecular weight of 65,740. An apparent leader sequence of 22 amino acids was observed. The promoter region for expression of the xylanase gene in Bacteroides species was identified with a promoterless chloramphenicol acetyltransferase gene. A region of high amino acid homology was found with the proposed catalytic domain of endoglucanases from several organisms, including Butyrivibrio fibrisolvens, Ruminococcus flavefaciens, and Clostridium thermocellum. The cloned xylanase was found to exhibit endoglucanase activity against carboxymethyl cellulose. Analysis of the codon usage for the xylanase gene found a bias towards G and C in the third position in 16 of 18 amino acids with degenerate codons.

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Year:  1993        PMID： 7763664     DOI： 10.1007/BF01576830

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  29 in total

1.  Introduction of the Bacteroides ruminicola xylanase gene into the Bacteroides thetaiotaomicron chromosome for production of xylanase activity.

Authors:  T R Whitehead; M A Cotta; R B Hespell
Journal:  Appl Environ Microbiol       Date:  1991-01       Impact factor: 4.792

2.  Terminators of transcription with RNA polymerase from Escherichia coli: what they look like and how to find them.

Authors:  V Brendel; G H Hamm; E N Trifonov
Journal:  J Biomol Struct Dyn       Date:  1986-02

3.  A new method for predicting signal sequence cleavage sites.

Authors:  G von Heijne
Journal:  Nucleic Acids Res       Date:  1986-06-11       Impact factor: 16.971

4.  Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

Authors:  C J Thompson; G S Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

5.  Structure and expression of the genes, mcrBDCGA, which encode the subunits of component C of methyl coenzyme M reductase in Methanococcus vannielii.

Authors:  D S Cram; B A Sherf; R T Libby; R J Mattaliano; K L Ramachandran; J N Reeve
Journal:  Proc Natl Acad Sci U S A       Date:  1987-06       Impact factor: 11.205

6.  Biochemical evidence that starch breakdown by Bacteroides thetaiotaomicron involves outer membrane starch-binding sites and periplasmic starch-degrading enzymes.

Authors:  K L Anderson; A A Salyers
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

7.  Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17.

Authors:  C Cunningham; C A McPherson; J Martin; W J Harris; H J Flint
Journal:  Mol Gen Genet       Date:  1991-08

8.  Heterologous expression of the Bacteroides ruminicola xylanase gene in Bacteroides fragilis and Bacteroides uniformis.

Authors:  T R Whitehead; R B Hespell
Journal:  FEMS Microbiol Lett       Date:  1990-01-01       Impact factor: 2.742

9.  Genetic analysis of a locus on the Bacteroides ovatus chromosome which contains xylan utilization genes.

Authors:  J Weaver; T R Whitehead; M A Cotta; P C Valentine; A A Salyers
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792

10.  Markedly unbiased codon usage in Bacillus subtilis.

Authors:  N Ogasawara
Journal:  Gene       Date:  1985       Impact factor: 3.688
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  9 in total

1.  Tracing determinants of dual substrate specificity in glycoside hydrolase family 5.

Authors:  Zhiwei Chen; Gregory D Friedland; Jose H Pereira; Sonia A Reveco; Rosa Chan; Joshua I Park; Michael P Thelen; Paul D Adams; Adam P Arkin; Jay D Keasling; Harvey W Blanch; Blake A Simmons; Kenneth L Sale; Dylan Chivian; Swapnil R Chhabra
Journal:  J Biol Chem       Date:  2012-05-29       Impact factor: 5.157

2.  Cloning, sequencing, and characterization of a membrane-associated Prevotella ruminicola B(1)4 beta-glucosidase with cellodextrinase and cyanoglycosidase activities.

Authors:  C R Wulff-Strobel; D B Wilson
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

3.  The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria.

Authors:  R G Gardner; J E Wells; J B Russell; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

4.  Use of a modified Bacteroides-Prevotella shuttle vector to transfer a reconstructed beta-1,4-D-endoglucanase gene into Bacteroides uniformis and Prevotella ruminicola B(1)4.

Authors:  R G Gardner; J B Russell; D B Wilson; G R Wang; N B Shoemaker
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

5.  Biochemical analysis of a beta-D-xylosidase and a bifunctional xylanase-ferulic acid esterase from a xylanolytic gene cluster in Prevotella ruminicola 23.

Authors:  Dylan Dodd; Svetlana A Kocherginskaya; M Ashley Spies; Kyle E Beery; Charles A Abbas; Roderick I Mackie; Isaac K O Cann
Journal:  J Bacteriol       Date:  2009-03-20       Impact factor: 3.490

6.  A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities.

Authors:  A Gasparic; J Martin; A S Daniel; H J Flint
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

7.  Molecular diversity of rumen bacterial communities from tannin-rich and fiber-rich forage fed domestic Sika deer (Cervus nippon) in China.

Authors:  Zhi Peng Li; Han Lu Liu; Guang Yu Li; Kun Bao; Kai Ying Wang; Chao Xu; Yi Feng Yang; Fu He Yang; André-Denis G Wright
Journal:  BMC Microbiol       Date:  2013-07-08       Impact factor: 3.605

8.  Secretory pathway of cellulase: a mini-review.

Authors:  Shaomin Yan; Guang Wu
Journal:  Biotechnol Biofuels       Date:  2013-12-02       Impact factor: 6.040

9.  Diet Transition from High-Forage to High-Concentrate Alters Rumen Bacterial Community Composition, Epithelial Transcriptomes and Ruminal Fermentation Parameters in Dairy Cows.

Authors:  Sonny C Ramos; Chang Dae Jeong; Lovelia L Mamuad; Seon Ho Kim; Seung Ha Kang; Eun Tae Kim; Yong Il Cho; Sung Sill Lee; Sang Suk Lee
Journal:  Animals (Basel)       Date:  2021-03-16       Impact factor: 2.752
  9 in total

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