Literature DB >> 18765909

Expression, purification and preliminary crystallographic analysis of N-acetylglucosamine-1-phosphate uridylyltransferase from Mycobacterium tuberculosis.

Jiang Yin1, Craig R Garen, Maia M Cherney, Leonid T Cherney, Michael N G James.   

Abstract

The gene product of open reading frame Rv1018c from Mycobacterium tuberculosis is annotated as encoding a probable N-acetylglucosamine 1-phosphate uridylyltransferase (MtbGlmU), an enzyme that catalyzes the biosynthesis of UDP-N-acetylglucosamine, a precursor common to lipopolysaccharide and peptidoglycan biosynthesis. Following overexpression in Escherichia coli, the enzyme was purified and crystallized using the hanging-drop vapour-diffusion method. Native diffraction data were collected from crystals belonging to space group R32 and processed to a resolution of 2.2 A.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18765909      PMCID： PMC2531282          DOI： 10.1107/S1744309108024500

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  20 in total

1.  ESPript: analysis of multiple sequence alignments in PostScript.

Authors:  P Gouet; E Courcelle; D I Stuart; F Métoz
Journal:  Bioinformatics       Date:  1999-04       Impact factor: 6.937

Review 2.  The TB structural genomics consortium: providing a structural foundation for drug discovery.

Authors:  Celia W Goulding; Marcin Apostol; Daniel H Anderson; Harindarpal S Gill; Clare V Smith; Mack R Kuo; Jin Kuk Yang; Geoffrey S Waldo; Se Won Suh; Radha Chauhan; Avinash Kale; Nandita Bachhawat; Shekhar C Mande; Jodie M Johnston; J Shaun Lott; Edward N Baker; Vickery L Arcus; David Leys; Kirsty J McLean; Andrew W Munro; Joel Berendzen; Vivek Sharma; Min S Park; David Eisenberg; James Sacchettini; Tom Alber; Bernhard Rupp; William Jacobs; Thomas C Terwilliger
Journal:  Curr Drug Targets Infect Disord       Date:  2002-06

3.  Structure of the Escherichia coli GlmU pyrophosphorylase and acetyltransferase active sites.

Authors:  L R Olsen; S L Roderick
Journal:  Biochemistry       Date:  2001-02-20       Impact factor: 3.162

4.  Crystal structure of the bifunctional N-acetylglucosamine 1-phosphate uridyltransferase from Escherichia coli: a paradigm for the related pyrophosphorylase superfamily.

Authors:  K Brown; F Pompeo; S Dixon; D Mengin-Lecreulx; C Cambillau; Y Bourne
Journal:  EMBO J       Date:  1999-08-02       Impact factor: 11.598

5.  Crystal structure of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase bound to acetyl-coenzyme A reveals a novel active site architecture.

Authors:  G Sulzenbacher; L Gal; C Peneff; F Fassy; Y Bourne
Journal:  J Biol Chem       Date:  2000-12-15       Impact factor: 5.157

6.  Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays.

Authors:  S V Gordon; R Brosch; A Billault; T Garnier; K Eiglmeier; S T Cole
Journal:  Mol Microbiol       Date:  1999-05       Impact factor: 3.501

7.  Crystal structures of Streptococcus pneumoniae N-acetylglucosamine-1-phosphate uridyltransferase, GlmU, in apo form at 2.33 A resolution and in complex with UDP-N-acetylglucosamine and Mg(2+) at 1.96 A resolution.

Authors:  D Kostrewa; A D'Arcy; B Takacs; M Kamber
Journal:  J Mol Biol       Date:  2001-01-12       Impact factor: 5.469

Review 8.  Mycobacterium tuberculosis: a model system for structural genomics.

Authors:  Clare V Smith; James C Sacchettini
Journal:  Curr Opin Struct Biol       Date:  2003-12       Impact factor: 6.809

9.  Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv.

Authors:  Jean-Christophe Camus; Melinda J Pryor; Claudine Médigue; Stewart T Cole
Journal:  Microbiology       Date:  2002-10       Impact factor: 2.777

Review 10.  The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology.

Authors:  T C Terwilliger; M S Park; G S Waldo; J Berendzen; L-W Hung; C-Y Kim; C V Smith; J C Sacchettini; M Bellinzoni; R Bossi; E De Rossi; A Mattevi; A Milano; G Riccardi; M Rizzi; M M Roberts; A R Coker; G Fossati; P Mascagni; A R M Coates; S P Wood; C W Goulding; M I Apostol; D H Anderson; H S Gill; D S Eisenberg; B Taneja; S Mande; E Pohl; V Lamzin; P Tucker; M Wilmanns; C Colovos; W Meyer-Klaucke; A W Munro; K J McLean; K R Marshall; D Leys; J K Yang; H-J Yoon; B I Lee; M G Lee; J E Kwak; B W Han; J Y Lee; S-H Baek; S W Suh; M M Komen; V L Arcus; E N Baker; J S Lott; W Jacobs; T Alber; B Rupp
Journal:  Tuberculosis (Edinb)       Date:  2003       Impact factor: 3.131
View more
  1 in total

1.  Expression, purification and preliminary crystallographic analysis of Rv3002c, the regulatory subunit of acetolactate synthase (IlvH) from Mycobacterium tuberculosis.

Authors:  Jiang Yin; Grace Garen; Craig Garen; Michael N G James
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-07-20
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.