| Literature DB >> 18722174 |
Silvestro G Conticello1, Karuna Ganesh2, Kanmin Xue2, Mason Lu2, Cristina Rada2, Michael S Neuberger3.
Abstract
Activation-induced deaminase (AID) deaminates deoxycytidine residues in immunoglobulin genes, triggering antibody diversification. Here, by use of two-hybrid and coimmunoprecipitation assays, we identify CTNNBL1 (also known as NAP) as an AID-specific interactor. Mutants of AID that interfere with CTNNBL1 interaction yield severely diminished hypermutation and class switching. Targeted inactivation of CTNNBL1 in DT40 B cells also considerably diminishes IgV diversification. CTNNBL1 is a widely expressed nuclear protein that associates with the Prp19 complex of the spliceosome, interacting with its CDC5L component. The results, therefore, identify residues in AID involved in its in vivo targeting and suggest they might act through interaction with CTNNBL1, giving possible insight into the linkage between AID recruitment and target-gene transcription.Entities:
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Year: 2008 PMID： 18722174 DOI： 10.1016/j.molcel.2008.07.009
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970