| Literature DB >> 18571452 |
Pedro J Batista1, J Graham Ruby, Julie M Claycomb, Rosaria Chiang, Noah Fahlgren, Kristin D Kasschau, Daniel A Chaves, Weifeng Gu, Jessica J Vasale, Shenghua Duan, Darryl Conte, Shujun Luo, Gary P Schroth, James C Carrington, David P Bartel, Craig C Mello.
Abstract
In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-RNA loci share a conserved upstream sequence motif, the mature 21U-RNAs are not conserved and, with few exceptions, fail to exhibit complementarity or evidence for direct regulation of other expressed sequences. Our findings demonstrate that 21U-RNAs are the piRNAs of C. elegans and link this class of small RNAs and their associated Piwi Argonaute to the maintenance of temperature-dependent fertility.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18571452 PMCID： PMC2570341 DOI： 10.1016/j.molcel.2008.06.002
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970