Literature DB >> 1841678

Construction of linear GlcNAc beta 1-6Gal beta 1-OR type oligosaccharides by partial cleavage of GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-OR sequences with jack bean beta-N-acetylhexosaminidase.

O Renkonen1, R Niemelä, A Leppänen, H Maaheimo, A Seppo, L Penttilä, A Vilkman.   

Abstract

Radiolabelled GlcNAc beta 1-3(GlcNAc beta 1-6)Gal (1), GlcNAc beta 1-3)GlcNAc beta 1-6)Gal beta 1-OCH3 (4), GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4Glc (7), and GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc (10) were cleaved partially with jack bean beta-N-acetylhexosaminidase (EC 3.2.1.30), and the digests were analysed chromatographically. All four oligosaccharides were hydrolysed faster at the (1-6) branch, than at the (1-3) branch, but a high branch specificity was observed only with the glycan 4. The saccharides 1 and 7 resembled each other in the kinetics of the enzyme-catalysed release of their two non-reducing N-acetylglucosamine units, but the glycan 10 was rather different. The partial digestions made it possible to obtain radiolabelled GlcNAc beta 1-6Gal, GlcNAc beta 1-6Gal beta 1-OCH3, GlcNAc beta 1-6Gal beta 1-4Glc, and, in particular, GlcNAc beta 1-6Gal beta 1-4GlcNAc.

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Year:  1991        PMID： 1841678     DOI： 10.1007/bf00731350

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  13 in total

1.  Branch specificity of beta-D-galactosidase from Escherichia coli.

Authors:  D H van den Eijnden; W M Blanken; A van Vliet
Journal:  Carbohydr Res       Date:  1986-08-15       Impact factor: 2.104

2.  The role of alpha-lactalbumin and the A protein in lactose synthetase: a unique mechanism for the control of a biological reaction.

Authors:  K Brew; T C Vanaman; R L Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1968-02       Impact factor: 11.205

3.  The linear tetrasaccharide, Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc, isolated from radiolabeled teratocarcinoma poly-N-acetyllactosaminoglycan resists the action of E. freundii endo-beta-galactosidase.

Authors:  O Renkonen; L Penttilä; A Makkonen; R Niemelä; A Leppänen; J Helin; A Vainio
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

4.  Escherichia coli beta-galactosidase unexpectedly cleaves the hexasaccharide Gal beta 1-4GlcNAc beta 1-3(Gal beta 1-4GlcNAc beta 1-6)Gal beta 1-4GlcNAc without branch specificity.

Authors:  O Renkonen; J Helin; A Vainio; R Niemelä; L Penttilä; P Hilden
Journal:  Biochem Cell Biol       Date:  1990 Jul-Aug       Impact factor: 3.626

5.  Wheat germ agglutinin chromatography of GlcNac beta 1-3(GlcNAc beta 1-6)Gal and GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc, obtained by in vitro synthesis and by partial cleavage of teratocarcinoma poly-N-acetyllactosaminoglycans.

Authors:  A Seppo; L Penttilä; A Makkonen; A Leppänen; R Niemelä; J Jäntti; J Helin; O Renkonen
Journal:  Biochem Cell Biol       Date:  1990-01       Impact factor: 3.626

6.  N-acetyllactosaminooligosaccharides that contain the beta-D-GlcpNAc-(1----6)-D-Gal or beta-D-GlcpNAc-(1----6)-D-GalNAc sequences reveal reduction-sensitive affinities for wheat germ agglutinin.

Authors:  O Renkonen; L Penttilä; R Niemelä; A Vainio; A Leppänen; J Helin; A Seppo; A Makkonen; H Maaheimo
Journal:  Carbohydr Res       Date:  1991-06-25       Impact factor: 2.104

7.  Mucin synthesis. III. UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase, an enzyme in porcine gastric mucosa involved in the elongation of mucin-type oligosaccharides.

Authors:  I Brockhausen; D Williams; K L Matta; J Orr; H Schachter
Journal:  Can J Biochem Cell Biol       Date:  1983-12

8.  Immobilized wheat germ agglutinin separates small oligosaccharides derived from poly-N-acetyllactosaminoglycans of embryonal carcinoma cells.

Authors:  O Renkonen; P Mäkinen; K Hård; J Helin; L Penttilä
Journal:  Biochem Cell Biol       Date:  1988-05       Impact factor: 3.626

9.  Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa.

Authors:  F Piller; J P Cartron; A Maranduba; A Veyrières; Y Leroy; B Fournet
Journal:  J Biol Chem       Date:  1984-11-10       Impact factor: 5.157

10.  Mucin synthesis. Conversion of R1-beta 1-3Gal-R2 to R1-beta 1-3(GlcNAc beta 1-6)Gal-R2 and of R1-beta 1-3GalNAc-R2 to R1-beta 1-3(GlcNAc beta 1-6)GalNAc-R2 by a beta 6-N-acetylglucosaminyltransferase in pig gastric mucosa.

Authors:  I Brockhausen; K L Matta; J Orr; H Schachter; A H Koenderman; D H van den Eijnden
Journal:  Eur J Biochem       Date:  1986-06-16
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  2 in total

1.  Single mid-chain GlcNAc beta 1-6Gal beta 1-4R sequences of linear oligosaccharides are resistant to endo-beta-galactosidase of Bacteroides fragilis.

Authors:  O Renkonen; L Penttilä; R Niemelä; A Leppänen
Journal:  Glycoconj J       Date:  1991-08       Impact factor: 2.916

2.  alpha 1,3-Fucosylation of branched blood group I-type oligo-(N-acetyllactosamino)glycans by human milk transferases is restricted to distal N-acetyllactosamine units: the resulting isomers are separated by WGA-agarose chromatography.

Authors:  R Niemelä; J Natunen; E Brotherus; A Saarikangas; O Renkonen
Journal:  Glycoconj J       Date:  1995-02       Impact factor: 2.916
  2 in total

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