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Literature DB >> 6231087

Mucin synthesis. III. UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase, an enzyme in porcine gastric mucosa involved in the elongation of mucin-type oligosaccharides.

I Brockhausen, D Williams, K L Matta, J Orr, H Schachter.

Abstract

Pig gastric mucosa microsomes have been shown to catalyze the following reaction: UDP-GlcNAc + Gal beta 1-3(GlcNAc beta 1-6)-GalNAc-alpha-R----GlcNAc beta 1-3Gal beta 1-3 (GlcNAc beta 1-6)GalNAc-alpha-R + UDP, where R is o-nitrophenyl or benzyl. The enzyme catalyzing this reaction has been named UDP-GlcNAc:Gal beta 1-3(GlcNAc beta 1-6)GalNAc-R (GlcNAc-R (GlcNAc to Gal) beta 3-N-acetylglucosaminyltransferase. The beta 3-GlcNAc-transferase does not act on Gal beta 1-3GalNAc-alpha-o-nitrophenyl. The beta 3-GlcNAc-transferase requires Mn2+ and Triton X-100 for optimal activity. The Vmax for the microsomal enzyme is 8.7 nmol/mg protein per hour and the Km values are 1.6, 0.9, and 1.2 mM for UDP-GlcNAc and the alpha-o-nitrophenyl and alpha-benzyl derivatives of Gal beta 1-3(GlcNAc beta 1-6)GalNAc, respectively. Pig gastric mucosa microsomes catalyze the transfer of GlcNAc to lactose to form GlcNAc beta 1-3Gal beta 1-4Glc, but fail to transfer GlcNAc to lactosyl ceramide, Gal beta 1-4GlcNAc, or Gal beta 1-4GlcNAc-beta-benzyl.

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Year: 1983 PMID： 6231087 DOI： 10.1139/o83-169

Source DB: PubMed Journal: Can J Biochem Cell Biol ISSN： 0714-7511

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Cited

8 in total

1. An approach for fluorometric determination of glycosyltransferase activities.

Authors: C A Palmerini; A Datti; I E Vanderelst; L Minuti; A Orlacchio
Journal: Glycoconj J Date: 1996-08 Impact factor: 2.916

Review 2. The joys of HexNAc. The synthesis and function of N- and O-glycan branches.

Authors: H Schachter
Journal: Glycoconj J Date: 2000 Jul-Sep Impact factor: 2.916

3. Preferred conformations and dynamics of five core structures of mucin type O-glycans determined by NMR spectroscopy and force field calculations.

Authors: A Pollex-Krüger; B Meyer; R Stuike-Prill; V Sinnwell; K L Matta; I Brockhausen
Journal: Glycoconj J Date: 1993-10 Impact factor: 2.916

4. Construction of linear GlcNAc beta 1-6Gal beta 1-OR type oligosaccharides by partial cleavage of GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-OR sequences with jack bean beta-N-acetylhexosaminidase.

Authors: O Renkonen; R Niemelä; A Leppänen; H Maaheimo; A Seppo; L Penttilä; A Vilkman
Journal: Glycoconj J Date: 1991-08 Impact factor: 2.916

8. UDP-GlcNAc: Gal beta 3GalNAc-mucin: (GlcNAc----GalNAc) beta 6-N-acetylglucosaminyltransferase and UDP-GlcNAc: Gal beta 3(GlcNAc beta 6) GalNAc-mucin (GlcNAc----Gal)beta 3-N-acetylglucosaminyltransferase from swine trachea epithelium.

Authors: S Sangadala; S Sivakami; J Mendicino
Journal: Mol Cell Biochem Date: 1991-03-13 Impact factor: 3.396