Literature DB >> 3408587

Immobilized wheat germ agglutinin separates small oligosaccharides derived from poly-N-acetyllactosaminoglycans of embryonal carcinoma cells.

O Renkonen1, P Mäkinen, K Hård, J Helin, L Penttilä.   

Abstract

Five pure oligosaccharides derived from poly-N-acetyllactosaminoglycans of teratocarcinoma cells were chromatographed on immobilized wheat germ agglutinin (WGA). Three of them, Gal beta 1-4GlcNAc, GlcNAc beta 1-3Gal, and GlcNAc beta 1-3Gal beta 1-4GlcNAc, revealed only weak binding, but GlcNAc beta 1-6Gal beta 1-4GlcNAc was bound moderately and GlcNAc beta 1-6Gal was bound quite strongly. The differences in the binding affinities were large enough to allow chromatographic separation of the five oligosaccharides into three distinct fractions in a small column of WGA-agarose.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3408587     DOI： 10.1139/o88-054

Source DB:  PubMed          Journal:  Biochem Cell Biol        ISSN： 0829-8211            Impact factor:   3.626


  7 in total

1.  The linear tetrasaccharide, Gal beta 1-4GlcNAc beta 1-6Gal beta 1-4GlcNAc, isolated from radiolabeled teratocarcinoma poly-N-acetyllactosaminoglycan resists the action of E. freundii endo-beta-galactosidase.

Authors:  O Renkonen; L Penttilä; A Makkonen; R Niemelä; A Leppänen; J Helin; A Vainio
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

2.  Carbohydrate binding specificities of several poly-N-acetyllactosamine-binding lectins.

Authors:  H Kawashima; S Sueyoshi; H Li; K Yamamoto; T Osawa
Journal:  Glycoconj J       Date:  1990       Impact factor: 2.916

3.  Structural requirements for the binding of oligosaccharides to immobilized lectin of Erythrina variegata (Linn) var. orientalis.

Authors:  H Li; K Yamamoto; H Kawashima; T Osawa
Journal:  Glycoconj J       Date:  1990       Impact factor: 2.916

4.  Construction of linear GlcNAc beta 1-6Gal beta 1-OR type oligosaccharides by partial cleavage of GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-OR sequences with jack bean beta-N-acetylhexosaminidase.

Authors:  O Renkonen; R Niemelä; A Leppänen; H Maaheimo; A Seppo; L Penttilä; A Vilkman
Journal:  Glycoconj J       Date:  1991-08       Impact factor: 2.916

5.  Oligo-N-acetyllactosaminoglycans bearing Gal beta 1-4(Fuc alpha 1-3)GlcNAc sequences reveal lower affinities than their nonfucosylated, or alpha(1-2) fucosylated counterparts for immobilized wheat germ agglutinin.

Authors:  O Renkonen; J Helin; L Penttilä; H Maaheimo; R Niemelä; A Leppänen; A Seppo; K Hård
Journal:  Glycoconj J       Date:  1991-08       Impact factor: 2.916

6.  Single mid-chain GlcNAc beta 1-6Gal beta 1-4R sequences of linear oligosaccharides are resistant to endo-beta-galactosidase of Bacteroides fragilis.

Authors:  O Renkonen; L Penttilä; R Niemelä; A Leppänen
Journal:  Glycoconj J       Date:  1991-08       Impact factor: 2.916

7.  alpha 1,3-Fucosylation of branched blood group I-type oligo-(N-acetyllactosamino)glycans by human milk transferases is restricted to distal N-acetyllactosamine units: the resulting isomers are separated by WGA-agarose chromatography.

Authors:  R Niemelä; J Natunen; E Brotherus; A Saarikangas; O Renkonen
Journal:  Glycoconj J       Date:  1995-02       Impact factor: 2.916
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.