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Literature DB >> 18370386

Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates.

Jae-Seung Lee¹, Dwight S Seferos, David A Giljohann, Chad A Mirkin.

Abstract

We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 +/- 6.4 pmol/cm2), and as a result, exhibit increased melting temperatures and cooperative melting properties.

Entities: Chemical

Mesh：

Substances：

Year: 2008 PMID： 18370386 PMCID： PMC8281389 DOI： 10.1021/ja800797h

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

26 in total

1. Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin.

Authors: Valeri Pavlov; Yi Xiao; Bella Shlyahovsky; Itamar Willner
Journal: J Am Chem Soc Date: 2004-09-29 Impact factor: 15.419

Review 2. Nanostructures in biodiagnostics.

Authors: Nathaniel L Rosi; Chad A Mirkin
Journal: Chem Rev Date: 2005-04 Impact factor: 60.622

3. A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes.

Authors: Abigail K R Lytton-Jean; Chad A Mirkin
Journal: J Am Chem Soc Date: 2005-09-21 Impact factor: 15.419

Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates.

1. Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin.

Review 2. Nanostructures in biodiagnostics.

3. A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes.

4. Gold and silica-coated gold nanoparticles as thermographic labels for DNA detection.

5. Temperature-programmed assembly of DNA:Au nanoparticle bioconjugates.

6. Organization of 'nanocrystal molecules' using DNA.

7. Thioalkylated tetraethylene glycol: a new ligand for water soluble monolayer protected gold clusters.

8. Oligonucleotide-modified gold nanoparticles for intracellular gene regulation.

9. "Coulomb Staircase" at Room Temperature in a Self-Assembled Molecular Nanostructure

10. Paclitaxel-functionalized gold nanoparticles.

Review 1. Nanoparticles containing insoluble drug for cancer therapy.

Review 2. Gold nanoparticles for biology and medicine.

3. Using DNA to Link Gold Nanoparticles, Polymers and Molecules: a Theoretical Perspective.

4. Aptamer-nanoparticle assembly for logic-based detection.

5. The role radius of curvature plays in thiolated oligonucleotide loading on gold nanoparticles.

6. Polyvalent DNA nanoparticle conjugates stabilize nucleic acids.

7. Aptamer nano-flares for molecular detection in living cells.

8. Engineering DNA-Functionalized Nanostructures to Bind Nucleic Acid Targets Heteromultivalently with Enhanced Avidity.