| Literature DB >> 18348468 |
Bedia Koçyiğit-Kaymakçioğlu1, Seda Unsalan, S Güniz Küçükgüzel, Göksel Sener, Sevim Rollas.
Abstract
The in vivo metabolism of 4-nitrobenzoic acid [(5-nitro-2-thiopheneyl)methylene]hydrazide (substrate), a model that represents hydrazide hydrazone compounds, was investigated in the rat. The metabolites were monitored in rat plasma at certain time intervals. The substrate was dissolved in dimethylsulfoxide (DMSO)/water (1:4) and administered intraperitoneally at dose of 100 mg/kg and 500 mg/kg. Blood samples were collected at 30 min, then at 1,2, 4, 8, 12 and 24 h post-administration. The chromatographic separation of the substrate and its metabolites was performed on aNovapak C18 (Phenomenex) (150 mm x 4.6 mm i.d., 5-microm particle size) using a mobile phase consisting of phosphate buffer: acetonitrile (90:10, v/v) with a linear gradient system. From the biotransformation of this compound, 4-nitrobenzoic acid (M3) was identified together with the substrate, as evidenced by high pressure liquid chromatography (HPLC)-UV/diode array detection (DAD).Entities:
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Year: 2007 PMID： 18348468 DOI： 10.1007/BF03191004
Source DB: PubMed Journal: Eur J Drug Metab Pharmacokinet ISSN： 0378-7966 Impact factor: 2.441