The safety profile of drotrecogin alfa (activated).

Continued safety assessment is essential for any newly approved therapy. Drotrecogin alfa (activated; DrotAA), which is approved for use in severe sepsis, has undergone clinical trials with corresponding safety analyses since 1995. However, the only comprehensive review of all trials is that reported in 2003 by Bernard and coworkers. This is an important review that focuses on the safety profile of DrotAA in all published studies (six randomized clinical trials and five national registry studies) and in previously unpublished data. DrotAA treatment is associated with an increased risk for bleeding (which in general is manageable). Nevertheless, the available evidence shows that any adverse effects of DrotAA are outweighed by its benefits in patients with severe sepsis who are at high risk for death. So far, more than 9,000 patients have been enrolled in clinical trials involving DrotAA, providing a valuable safety database. It is of note that although DrotAA does increase the risk of bleeding, this has not been associated with an overall increase in the rate of all severe adverse events.

Introduction

Drotrecogin alfa (activated; DrotAA) is the first and thus far only compound proven to improve survival in patients with severe sepsis. The mechanisms that underlie the improved survival are not entirely clear. A number of studies have demonstrated that DrotAA exerts an anticoagulant effect through inactivation of coagulation factors Va and VIIIa [1], an anti-inflammatory effect [2] and a profibrinolytic effect [3], as well as antiapoptotic [4] and cytoprotective effects [5]. Of these, the anticoagulant property is the action associated with the only known adverse effect of treatment, namely an increased risk for bleeding.

Continued assessment of safety is essential in any newly approved therapy. DrotAA has been studied in clinical trials since 1995 (Table 1). It was approved for use in adult patients with severe sepsis at high risk of death in the USA in November 2001 [6] and in the European Union in August 2002 [7]. The European Medicines Agency indicates that DrotAA may be used in the treatment of adult patients with severe sepsis and multiple organ failure, and reaffirmed this indication as a result of the second annual licence reassessment, stating that DrotAA should be considered mainly in situations in which therapy can be initiated within 24 hours after the onset of organ failure [8].

Table 1

Drotrecogin alfa (activated) clinical trials

Study	Study type/patients or treatments included	Number of patients included	Study period	Reference
EVAA	Dose-finding phase II	131	1997 to 1998	[10]
PROWESS	Phase III	1,690	1998 to 2000	[11]
ENHANCE	Open-label	2,378	2001 to 2003	[12]
ADDRESS	Low risk for death	2,640	2002 to 2004	[13]
RESOLVE	Sepsis in children	477	2002 to 2005	[24]
XPRESS	Heparin + DrotAA	1,927	2003 to 2005	[14]

ADDRESS, Efficacy and Safety of Drotrecogin alfa [activated] in Adult Severe Sepsis Patients at Low Risk of Death; DrotAA, drotrecogin alfa (activated); ENHANCE, Extended Evaluation of Recombinant Human Activated Protein C, drotrecogin alfa (activated); EVAA, [dose finding study]; PROWESS, Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis; RESOLVE, REsearching severe Sepsis and Organ dysfunction in children: a gLobal perspective; XPRESS, Xigris and Prophylactic Heparin Evaluation in Severe Sepsis.

From the very first clinical study, safety analyses have been included in each controlled trial conducted in DrotAA, and continuous safety surveillance has formed part of all studies undertaken for the purposes of registering the drug for commercial use. In this review we analyze the available literature regarding the safety of DrotAA, together with some previously unpublished data.

Safety profile in clinical trials

In the early phase I studies, begun more than 10 years ago, adverse events with a frequency of 5% or greater included headache (30.9%), ecchymosis (23.0%), and pain (5.8%), primarily at the site of venopuncture [9]. These studies were conducted in healthy volunteers mainly to evaluate pharmacokinetics, and no further safety issues were identified to prevent future clinical development [9]. The data regarding serious bleeding events are summarized in Table 2. The definition of a serious bleeding event includes any intracranial hemorrhage, any life-threatening or fatal bleed, any bleeding event requiring the administration of three or more units of packed red blood cells per day for 2 consecutive days, or any bleeding event considered serious by the investigator. A similar definition was adopted for all DrotAA studies except for ADDRESS and XPRESS, in which requirements for transfusion were not included.

Table 2

Serious adverse events including bleeding events through clinical trials

	Placebo-controlled studies				Open-labela
	PROWESS		ADDRESS		ENHANCE	XPRESS
Adverse events	DrotAA (n = 850)	Placebo (n = 840)	DrotAA (n = 1,317)	Placebo (n = 1,293)	DrotAA (n = 2,378)	DrotAA (n = 1,935)
Study drug infusion period*
Serious events	58 (6.8)	55 (6.5)	75 (5.7)	78 (6.0)	166 (7.0)	128 (6.6)
Serious bleeds	20 (2.4)	8 (1.0)	31 (2.4)	15 (1.2)	85 (3.6)	46 (2.4)
CNS bleeds	2 (0.2)	0	4 (0.3)	3 (0.2)	15 (0.6)	6 (0.3)
Days 0 through 28
Serious events	106 (12.5)	102 (12.1)	182 (13.8)	183 (14.2)	319 (13.4)	256 (13.2)
Serious bleeds	30 (3.5)	17 (2.0)	51 (3.9)	28 (2.2)	155 (6.5)	88 (4.5)
CNS bleeds	2 (0.2)	1 (0.1)	6 (0.5)	5 (0.4)	35 (1.5)	17 (0.9)

*In PROWESS and ENHANCE, the study drug infusion period was defined as the actual infusion plus 1 day. In ADDRESS, RESOLVE and XPRESS, the study drug infusion period was defined as study days 0 through 6. Values are expressed as n (%). aENHANCE was an open-label study. XPRESS was a placebo-controlled study of the co-administration of heparin with drotrecogin alfa (activated); the drug under study was heparin; open-label drotrecogin alfa (activated) was administered to all patients. ADDRESS, Efficacy and Safety of Drotrecogin alfa [activated] in Adult Severe Sepsis Patients at Low Risk of Death; CNS, central nervous system; DrotAA, drotrecogin alfa (activated); ENHANCE, Extended Evaluation of Recombinant Human Activated Protein C, drotrecogin alfa (activated); PROWESS, Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis; XPRESS, Xigris and Prophylactic Heparin Evaluation in Severe Sepsis. Modified with permission from Williams MD, Macias W, Rustige J: Safety of drotrecogin alfa (activated): a fair comparison requires consistent definitions. Intensive Care Med 2007, 33:1487–1488. © Springer-Verlag.

The first patients with severe sepsis to receive DrotAA were those included in the EVAA (dose finding) study [10]. This was a proof-of-concept study that investigated the safety profile of DrotAA and determined the optimal dosage for future trials. Four (4%) patients receiving DrotAA and two (5%) patients receiving placebo during the 28-day period of study reported serious bleeding events. Two events, haematemesis and worsening haematoma, occurred during DrotAA infusion and the other two, worsening gastrointestinal hemorrhage and hemorrhage during laparotomy, occurred 1 and 25 days after infusion. The two gastrointestinal hemorrhages in the control group occurred 3 and 4 days after placebo infusion.

In EVAA, overall, there was no statistically significant difference between DrotAA-treated and placebo-treated patients experiencing at least one serious adverse event (39% versus 46%, respectively; P = 0.422). Skin rash occurred more frequently among DrotAA-treated than placebo-treated patients (10% versus 0%, respectively; P = 0.036). None of the rashes was classified as serious or considered by the investigators to be drug related. No significant differences in specific treatment-emergent adverse events were recorded and no safety issues were identified in central laboratory parameters or vital signs.

For 53 treated patients, samples for anti-DrotAA antibody testing were available. A low-level anti-DrotAA antibody response was reported in one patient; the antibodies were non-neutralizing with respect to the anticoagulant activity. This patient did not experience any related clinical adverse events and was negative for anti-DrotAA antibody response 1 year after exposure.

These EVAA results were sufficiently encouraging to proceed to the pivotal phase III double-blind, randomized, placebo-controlled PROWESS (Recombinant Human Activated Protein C Worldwide Evaluation in Severe Sepsis) trial.

PROWESS, ENHANCE, ADDRESS, and XPRESS

These four studies used all the same dose of DrotAA (24 μg/kg per hour), which was the one that was demonstrated to be more effective and safe in EVAA.

The pivotal phase III PROWESS trial [11] included 850 DrotAA-treated and 840 placebo-treated patients. The percentages of patients experiencing at least one serious adverse event were 12.1% in the DrotAA group and 12.5% in the placebo group (P = 0.84), whereas percentages of those experiencing at least one bleeding event were 24.9% in the DrotAA group and 17.7% in the placebo group (P < 0.05). The majority of bleeding events in both groups were ecchymosis or gastrointestinal tract bleeding and were not considered serious. The difference in the incidence of serious bleeding events between the two treatment groups occurred primarily during drug administration.

In ENHANCE (Extended Evaluation of Recombinant Human Activated Protein C, drotrecogin alfa [activated]), a single-arm, open-label trial [12], a total of 2,378 adult patients with severe sepsis received DrotAA. Given that the rate of serious bleeding events was also higher in ENHANCE during the post-infusion period, where, due to the short half-life of the drug, patients were not exposed to DrotAA, this suggests a higher baseline risk for bleeding in ENHANCE compared with PROWESS, with higher degrees of both haematologic and hepatic dysfunction, both of which are associated with increased bleeding risk. The incidences of serious bleeding events in both the infusion and post-infusion periods in the PROWESS and ENHANCE studies are summarized in Table 2.

The ADDRESS (Efficacy and Safety of Drotrecogin alfa [activated] in Adult Severe Sepsis Patients at Low Risk of Death) study [13] involved 1,317 DrotAA-treated and 1,293 placebo-treated patients. The percentages of patients experiencing at least one bleeding event in the DrotAA and placebo treatment groups were 10.9% and 6.4%, respectively (P < 0.001). The numbers of serious bleeding events during infusion (defined as study day 0 through to study day 6) were 31 (2.4%) in DrotAA-treated and 15 (1.2%) in placebo-treated patients (P = 0.02). The numbers of patients suffering central nervous system (CNS) bleeding during infusion were 4 (0.3%) and 3 (0.2%) in the DrotAA and placebo groups, respectively. Recent surgery (within 30 days before study entry) was associated with a greater risk for serious bleeding during infusion in both the DrotAA-treated and placebo-treated patients (3.6% versus 1.6% in DrotAA-treated patients with and without recent surgery, respectively; and 1.6% versus 0.9% placebo-treated patients with and without recent surgery, respectively); however, this difference between groups was not significant.

XPRESS (Xigris and Prophylactic Heparin Evaluation in Severe Sepsis) [14] was an open label study, all the patients received DrotAA and the study drug was prophylactic heparin or placebo. It included patients with severe sepsis and greater disease severity (for example, a higher rate of organ dysfunctions) than in previous studies (because it was performed in an on-label population), who were undergoing treatment with DrotAA. Serious bleeding rates were consistent with those observed in previous studies throughout the 6-day treatment period, and prophylactic heparin did not increase the risk for serious bleeding compared with placebo (2.3% and 2.5%, respectively), including CNS bleeding (0.3% in both arms). However, the group receiving prophylactic heparin had a significantly higher rate of nonserious bleeding in comparison with the placebo group (8.7% versus 5.7%, respectively; P = 0.0116).

Serious bleeding events during the 28-day study period

In PROWESS the incidence of serious bleeding events during the 28-day study period was 3.5% in DrotAA-treated and 2.0% in placebo-treated patients. The incidences of CNS bleeds during this period were 0.2% and 0.1% for DrotAA-treated and placebo-treated patients, respectively. The risk for CNS bleeding may increase with severe coagulopathy and severe thrombocytopenia [15]. In ADDRESS the corresponding results were 3.9% in DrotAA-treated and 2.2% in placebo-treated patients (P = 0.01), and 0.5% and 0.4%, respectively, for CNS bleeds.

Serious bleeding rates in XPRESS [14] were consistent with those observed in previous studies during the 28-day period of study. Prophylactic heparin did not increase the risk for serious bleeding for DrotAA compared with placebo (3.9% versus 5.2%, respectively), including CNS bleeding (1.0% versus 0.7%, respectively). In ENHANCE, 6.5% of DrotAA-treated patients had serious bleeding events during days 0 to 28, and the incidence of CNS bleeds was 1.5%.

Safety profile outside controlled clinical trials

Two years after DrotAA had been approved by the US Food and Drug Administration for use in adult patients with severe sepsis at high risk of death, Bernard and coworkers [16] reported the first post-marketing safety assessment, and at least five National Registries have been published to date [17-21].

Although all of the referenced studies [17-21] carried out a safety analysis, the findings are not entirely comparable because diverse definitions of bleeding were employed in the different registries and these were sometimes different from those used in the controlled clinical trials. Table 3 summarizes the registries that included safety findings.

Table 3

Safety profile in national registries

	UK [17]	Poland [19]	Italy [20]	Canada [21]
Patients (n)	1279	302	324	261
Adverse events (%)	7.4	N/A	N/A	N/A
Bleeding (%)	5.9	6	9	N/A
Serious bleeding (%)	N/A	1.7	4	10

Only the four published national registries in which safety analyses were consistent or part of the objectives of the study are listed here. Note that different definitions were used for bleeding and serious bleeding among registries, and these were also different between registries and controlled clinical trials. N/A = Not available.

Safety profile in special populations

Surgical patients

Surgical patients with severe sepsis account for 25% to 50% of patients treated in randomized clinical trials and registry studies of DrotAA. The main complication after major surgery is bleeding, and therefore this group of patients represents a special population from a safety standpoint. The main analysis of treated patients published so far concluded that although patients receiving DrotAA are at greater risk for bleeding, the risk-benefit profile still favors use of DrotAA in patients in whom it is indicated [22].

The PROWESS Trial Surgical Evaluation Committee on efficacy and safety of DrotAA in surgical patients confirmed 28% of cases as surgical. Barie et al. [22], moreover, report that for all surgical patients and those undergoing intraabdominal procedures, the absolute risk reduction for mortality was 3.2% and 9.1%, respectively. Administration of DrotAA was associated with an increased risk for serious bleeding during the infusion period in both the overall PROWESS patients (P = 0.024) and the surgical cohort (P = 0.006); however, the surgical patients did not demonstrate excessive risk of bleeding compared with nonsurgical patients who received DrotAA. Serious bleeding during the infusion and 28-day period was similar between surgical and nonsurgical patients. For the three patient populations PROWESS overall, PROWESS Surgical and PROWESS non-Surgical, respectively, the serious bleeding event rates were 2.4% versus 3.1% versus 2.1% during the infusion period, and 3.5% versus 3.5% versus 3.5% during the 28-day period. In ADDRESS, recent surgery (within 30 days prior to study entry) was associated with a numerically higher risk of serious bleeding during infusion in both the Xigris-treated and the placebo-treated patients (Xigris: 3.6% in patients with recent surgery versus 1.6% in patients without recent surgery; placebo: 1.6% versus 0.9%, respectively).

Among the small number of patients (98) enrolled in PROWESS with single organ dysfunction and recent surgery (surgery within 30 days prior to study treatment), 28-day all-cause mortality was 10/49 versus 8/49 for the Xigris and placebo patients, respectively (*P = 0.60); the in-hospital mortality was 14/48 versus 8/47 for the Xigris and placebo patients, respectively (*P = 0.16). The subset of patients (636) with single organ dysfunction and recent surgery from the randomized, placebo-controlled study (ADDRESS) of septic patients at lower risk of death (Acute Physiology and Chronic Health Evaluation [APACHE] II score <25 or single sepsis-induced organ failure at any APACHE II score) had a 28-day all-cause mortality of 67/323 versus 44/323 for Xigris and placebo patients, respectively (*P = 0.03) and an in-hospital mortality of 76/325 versus 62/314 for Xigris and placebo patients, respectively (*P = 0.26). (*Chi-square test without adjustment for multiple comparisons.)

The incidence of serious bleeding events in patients with single organ dysfunction and recent surgery reported in PROWESS during infusion was 4.2% versus 0% for Xigris and placebo patients, respectively (P = 0.16) and during the 28-day period was 4.2% versus 0% for Xigris and placebo patients, respectively (P = 0.16); in ADDRESS the incidence of serious bleeding events during infusion was 4.4% versus 1.9% for Xigris and placebo patients, respectively (P = 0.08), and for 28 days 5.0% versus 2.9% for Xigris and placebo patients, respectively (P = 0.17).

DrotAA is not indicated for patients with single organ dysfunction and in particular should not be used in patients with single organ dysfunction and recent surgery.

Recommendations for patients under interventions

When surgery or other invasive procedures are performed during the infusion of DrotAA, the recommendations for interruption of infusion should be followed to minimize the risk for bleeding. Pharmacokinetic studies show that DrotAA has a short half-life. Plasma concentrations decline rapidly, with no measurable activity within 2 hours after the infusion is stopped.

As stated in the current European Summary of Product Characteristics, 'For procedures with an inherent bleeding risk, discontinue drotrecogin alfa (activated) for 2 hours prior to the start of the procedure. Drotrecogin alfa (activated) may be restarted 12 hours after major invasive procedures or surgery if adequate haemostasis has been achieved. Drotrecogin alfa (activated) may be restarted immediately after uncomplicated less invasive procedures if adequate haemostasis has been achieved.' [8].

The decision regarding the interruption/post-procedure reinstitution of DrotAA for surgical procedures should be made by the attending clinician on a case-by-case basis after thorough assessment of benefit versus risk. Table 4[23] provides guidelines for interruption and eventual restart of DrotAA in patients treated invasively.

Table 4

Guidelines for interrupting drotrecogin alfa (activated) infusion for an invasive procedure

Procedurea	Pre-procedure: stop DrotAA	Post-procedure: reconsider DrotAAb
Minor procedure
Arterial line insertion	2 hours before procedure	Immediately afterward
Venous femoral line insertion	2 hours before procedure	Immediately afterward
Re-intubation (tube change)	2 hours before procedure	Immediately afterward
More invasive procedure
Central venous catheter insertion via compressible site (e.g. via femoral vein)	2 hours before procedure	Immediately afterward
Central venous catheter insertion via noncompressible site (e.g. via subclavian or jugular vein)	2 hours before procedure	2 hours afterward
Chest tube insertion or thoracic drainage	2 hours before procedure	2 hours afterward
Gastroscopy (possible biopsy)	2 hours before procedure	2 hours afterward
Lumbar puncture	2 hours before procedure	2 hours afterward
Nephrostomy	2 hours before procedure	2 hours afterward
Paracentesis	2 hours before procedure	2 hours afterward
Percutaneous drainage	2 hours before procedure	2 hours afterward
Sinus puncture	2 hours before procedure	2 hours afterward
Tracheostomy	2 hours before procedure	1 hour afterward
Wound debridement (decubitus ulcer, infected wound, packing changes in open abdomen, etc.)	2 hours before procedure	2 hours afterward
Major procedure
Major surgical procedurec	2 hours before procedure	Wait 12 hours
Epidural catheter	2 hours before procedure; withhold drotrecogin alfa (activated) while catheter present	Wait at least 12 hours after catheter removal before initiating drotrecogin alfa (activated) infusion
Other
CPR	When beginning CPR	Evaluate neurologic status before resuming drotrecogin alfa (activated). If chest trauma occurred, wait 12 hours

aThese guidelines assume that the procedures listed (with the exception of initiation of cardiopulmonary resuscitation [CPR]) are non-emergency. For emergency procedures, the drotrecogin alfa (activated; DrotAA) infusion should be discontinued as soon as possible. The subsequent decision regarding whether and when to reinitiate the infusion depends on the type of procedure and the patient's clinical condition. bIf no signs and symptoms of bleeding are present and the investigator/clinician feels that there is minimal risk for bleeding complications. cA significant surgical procedure that requires the use of an operating room, anesthesia, and so on. DrotAA, drotrecogin alfa (activated). Modified with permission from Laterre and Wittebole [23].

Pediatric patients

DrotAA is not recommended for use in persons under the age of 18 years and should not therefore be used in these patients. From a safety point of view, in the RESOLVE (REsearching severe Sepsis and Organ dysfunction in children: a gLobal perspective) study [24], a double-blind, placebo-controlled trial conducted in children with severe sepsis, the investigators attributed two deaths in the DrotAA group and five in the placebo group to bleeding events. There was a higher rate of CNS bleeding in the DrotAA group than in the placebo group. Over the infusion period (study days 0 to 6), the number of patients experiencing CNS bleeding was 5 (2.1%) in the DrotAA group compared with 1 (0.4%) in the placebo group, with four of the five events in the DrotAA group occurring in children aged 60 days or younger, or with a body weight of 3.5 kg or less. Fatal CNS bleeding events, serious bleeding events (over the infusion period and over the 28-day study period), serious adverse events, and amputations were similar in the DrotAA and placebo groups.

Interaction with other drugs

In the XPRESS trial [14] a total of 1,935 adult patients with severe sepsis treated with DrotAA were randomly assigned to receive either prophylactic heparin or placebo. Prophylactic heparin doses used in XPRESS were 10,000 U/day unfractionated heparin or 40 mg/day enoxaparin administered subcutaneously. The subgroup of patients whose baseline heparin was discontinued at randomization exhibited increased mortality and serious adverse events, including cardiac, gastrointestinal, and venous thrombotic events. Therefore, prophylactic heparin should not be discontinued before initiating treatment or during treatment with DrotAA, unless it is considered medically necessary for other reasons. Starting treatment with DrotAA does not in itself require cessation of prophylactic heparin.

In 2004, Mann and coworkers [25] reported an observation on the compatibility of DrotAA with 34 other very commonly used therapies when infused through a 'Y' site infusion device. Of the 34 test drugs, eight were defined as visually compatible with DrotAA.

Immunogenicity and re-administration

In patients with severe sepsis, the formation of anti-DrotAA antibodies was uncommon (<1%) after a single course of therapy. These antibodies were not capable of neutralizing the effect of activated protein C in the activated partial thromboplastin time assay. However, the possibility of allergic reactions to constituents of the preparation cannot be completely excluded in certain predisposed patients [8]. sick individuals, even after repeated administration. DrotAA re-administration is not contraindicated [8]; a few anecdotal reports describe repeated re-administration in a clinical setting [26].

Discussion

Clinical trials with DrotAA have now been performed for longer than 10 years and all have shown the same pattern in terms of safety. DrotAA increases the risk for bleeding without increasing general adverse events, thus resulting in similar rates of serious adverse events in treated arms compared with placebo.

Other types of adverse events (risk for superinfection, formation of blocking antibodies) were prospectively measured in randomized controlled trials and the results were consistent across trials. The only adverse event attributable to DrotAA is an increased risk for bleeding. Serious adverse bleeding events in randomized trials occurred at consistently low rates, as shown in Table 2.

The increase in the number of bleeding events compared with placebo can be explained given the mechanism of action of DrotAA. The risk for bleeding can be reduced by following simple guidelines and avoiding off-label use (use in contraindicated patients). It is important to note that the intracranial hemorrhage rate in all of the studies is in accordance with the rate of spontaneous intracranial hemorrhage in critically ill patients [27]. Moreover, some studies have suggested that the rate of intracranial hemorrhage may be much higher in patients with septic shock. In one study, autopsy examination revealed evidence of cerebral hemorrhage in six out of 23 septic shock patients (26%) [15].

In other settings, such as acute cardiac syndrome, the use of thrombolytic or antiplatelet compounds carry an inherent risk for bleeding that is even greater than that observed with DrotAA, but nevertheless this risk is considered acceptable.

It has been postulated that the anticoagulant effect of DrotAA may play a less important role in increasing survival rate in severe sepsis patients at high risk for death. Hence, development of a new form of the therapy with a reduced anticoagulant effect, while conserving its other properties, might enhance the benefit-risk ratio [28]. Nevertheless, when comparing the risk for bleeding in DrotAA-treated patients with the risk in those enrolled in trials of other natural anticoagulants, the benefit-risk ratio is greater in DrotAA patients [29,30].

A recently published long-term follow up of ADDRESS [31] concluded that DrotAA exerts no harmful effects in patients with severe sepsis who are at low risk for death. Also, because of the drug's pharmacokinetic characteristics, any such effects would be limited to the infusion period, whereas its benefits persist in the population for which it is indicated.

Numerous registries have been reported to date. The conclusions of all of these studies recognize that DrotAA is the only compound to have demonstrated any effect on survival in septic patients and that there is no evidence that risk outweighs benefit when it used within the indicated population [32]. Efforts should be focused on education and adequate patient identification [21], especially in the setting of sepsis, where abnormal coagulation is the rule [33].

Conclusion

Although DrotAA increases the risk for bleeding, the safety profile remains acceptable for the indicated population after more than 10 years of clinical research. In other words, the benefit to patients at high risk for death is not outweighed by the adverse effects of DrotAA [34]. The increased risk for bleeding can be minimized by following simple guidelines and through appropriate patient selection.

Abbreviations

CNS = central nervous system; DrotAA = drotrecogin alfa (activated).

Competing interests

RF participated as Principal Investigator in Eli Lilly and Company sponsored trials, received speaker's fees from Eli Lilly and Company, and is member of their Advisory Board. He received no funds for writing this review. MAM is full-time employee of Eli Lilly and Company.

Authors' contributions

RF and MAM participated in the conception and design of the review, the acquisition of the data from the literature and the interpretation of the data, and drafted the manuscript. Both also participated in the analysis and interpretation of the data, critically revised the manuscript for important intellectual content, and gave final approval of the version to be published.