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Literature DB >> 18188474

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.

William P Heal¹, Sasala R Wickramasinghe, Paul W Bowyer, Anthony A Holder, Deborah F Smith, Robin J Leatherbarrow, Edward W Tate.

Abstract

N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.

Entities: Chemical

Mesh：

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Year: 2007 PMID： 18188474 DOI： 10.1039/b716115h

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

27 in total

1. Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry.

Authors: William P Heal; Megan H Wright; Emmanuelle Thinon; Edward W Tate
Journal: Nat Protoc Date: 2011-12-22 Impact factor: 13.491

2. Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.

Authors: John P Wilson; Anuradha S Raghavan; Yu-Ying Yang; Guillaume Charron; Howard C Hang
Journal: Mol Cell Proteomics Date: 2010-11-14 Impact factor: 5.911

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.

1. Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry.

2. Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.

Review 3. Enzymatic labeling of proteins: techniques and approaches.

4. Evaluation of alkyne-modified isoprenoids as chemical reporters of protein prenylation.

5. Recent advances in chemical proteomics: exploring the post-translational proteome.

Review 6. Exploring protein lipidation with chemical biology.

Review 7. Biomolecular engineering for nanobio/bionanotechnology.

8. Bioorthogonal chemical reporters for analyzing protein lipidation and lipid trafficking.

9. A Method to Generate and Analyze Modified Myristoylated Proteins.

10. Lipid modification of proteins through sortase-catalyzed transpeptidation.